左西孟旦对大鼠离体心脏缺血再灌注损伤的保护作用 .pdfVIP

左西孟旦对大鼠离体心脏缺血再灌注损伤的保护作用

于明懂;高春霖;吕国义;邓廼封

【摘要】目的:观察含左西孟旦(Levo)的停搏液对大鼠离体缺血再灌注心肌的保护

作用.方法:32只雄性Wistar大鼠随机分为10、L0.03、L0.3、LG共4组,每组8

只,在去极化停搏液STH-2中分别加入Levo0、0.03、0.3μmol/L以及Levo0.3

μmol/L+格列苯脲10μmol/L.平衡灌注30min后分别用25℃的4种不同停搏液

停搏,2h后重新灌注30min,观察对比各组停搏前后不同时间点心功能指标、再灌

注末冠脉流出液中乳酸脱氢酶(LDH)和肌酸激酶(CK)含量的不同.结果:各组心功能

基础值差异无统计学意义(P＞0.05),再灌注期各组心功能都有不同程度的降低,L0.3

组左心室舒张末压(LVDP)、心室内压力上升/下降速率(±dp/dtmax1的恢复率均

高于其他3组(P＜0.01或P＜0.05),HR的恢复率明显高于L0组(p＜0.01),L0.03

组、LG组与L0组比较心率(HR)、+dp/dtmax的恢复率差异无统计学意义(P＞

0.05),-dp/dtmax恢复率在T2时点高于L0组(P＜0.05或P＜0.01).再灌注末CK

和LDH漏出量L0.3组明显低于其他各组(P＜0.05或P＜0.01).结论:Levo加入

STH-2停搏液能够明显减轻缺血再灌注心肌的损伤.%Objective:Toobservethe

protectiveeffectoflevosimendan(Levo)incardioplegicsolutionon

isolatedratheart.Methods:Thirty-twomaleWistarratswererandomly

dividedintofourgroups:noLevo(LO)group,0.03μmol/LLevo(L0.03)

group,0.3μmol/LLevo(L0.3)group,0.3μmol/LLevoand10μmol/L

glibenclamide(LG)group.TheheartswasconnectedtoLangendorff

perfusionapparatusimmediatelyafterperfusedwithK-Hsolutionfor30

minutes.Heartswerethenperfusedbydifferentcardioplegicsolutionsat

25℃for2h,andthenwerereperfusedwithK-Hsolutionfor30minutes.

Thechangesofhemodynamics,myocardialenzymeleakageinthe

coronaryeffluentwereobserved.Results:Therewerenosignificant

differencesinhemodynsmics,creatinekinase(CK)andlactate

dehydrogenase(LDH)beforeheartsstoppedbeatinginallgroups(P

0.05).Afterreperfusion,therecoveryvaluesofheartrate(HR),left

ventriculardevelopedpressure(LVDP)andtherateofpressure

development(±dp/dumax)werehigheringroupL0.3thanthatofgroupL0

(P0.01).Therewerenosignificantdifferencesintherecoveryvaluesof

HRand±dp/du?BetweengroupsL0.03,LGandL0(P0.05).Therecovery

of±dp/dtmax,washigheratT2ingroupsL0.03andLGthanthatofgroup