α-硫辛酸对高盐诱导的大鼠高血压反应过程的抑制作用 .pdfVIP

α-硫辛酸对高盐诱导的大鼠高血压反应过程的抑制作用

胥艳;袁丹;刘东亮;王宇;朱蜀侠;苟伟

【摘要】ObjectiveToinvestigatewhethertheexcessiveproductionof

reactiveoxygenspecies(ROS)inthemitochondriaoftherostral

ventrolateralmedulla(RVLM)caninhibitthehighsalt-induced

hypertensionresponse.MethodsAtotaloff32maleratsweredividedinto

twogroups:twogroupsweregivennormalsaltdiet(0.3％NaCl)for8

weeks(n=16)andhighsaltdiet(8％NaCl)for8weeks(n=16,induced

hypertensionmodel)respectively.Thetwogroupsweredividedintofour

groups,twogroupsweregivenα-lipoicdissolvingin0.9％normalsaline

(60mg/kg),twogroupswerefedwithsalinefor9weeks.Therewereffour

groups:theexperimentalgroup(n=8,thehypertensionmodelsamplefed

α-lipoicacid),themodelgroup(n=8,thehypertensionmodelsamplefed

saline),thecontrolgroup(n=8,normalsaltdietsamplefedα-lipoicacid)

andtheblankcontrolgroup(n=8,normalsaltdietsampleffed

saline).Monitoredthechangeofthearterialpressureanddetectedthe

expressionoffsuperoxidebyimmunofluorescenceattheendofthe

experiment,measuredtheexpressionofNAD(P)HNOX2,NOX4andCu/Zn-

SODinRVLMbyWesternblot;determinedtheexpressiondifferencesof

oxidativestressrelatedsubstancessuchasmitochondrial

malondialdehyde(MDA)inRVLMbyELISA.ResultsThemeanarterial

pressure(MAP)intheexperimentalgroupwaslowerthanthatinthe

modelgroup,thedifferencewasstatisticallysignificant(P＜0.05);inthe

experimentalgroupandthemodelgrouptheintensitiesoffluorescent-

lableddihydroethidium(DHE)were60.2±3.1,99.1±3.8;thenumbersof

positiveneuronsinCu/Zn-SODwere20.8±1.1,6.9±1.2;thenumbersof

NOX2positiveneuronswere12.3±3.5,25.1±5.4;thenumbersofNOX4

positiveneuronswere10.1±2.2,13.3±4.1,thedifferencewasstatistically

significant(P＜0.05).WesternblotshowedthattheNOX2levelsofthe

experimentalgroupandthemodelgroupwere78.9±2.0,112.7±3.8;the

leve