《Xiayuxue Decoction （下瘀血汤） Attenuates Hepatic Stellate Cell Activation and Sinusoidal Endothelium Defenestration in CCI4-1nduced Fibrotic Liver of Mice.》.pdf

16． · 516· ORIGINALARTICLE XiayuxueDecoction(下瘀血汤)AttenuatesHepaticStellate CelIActivationandSinusoidalEndothelium DefenestratiOninCCI4-InducedFibroticLiverofMice ZHANGLi．jun(章李军)’，SUNMing．yu(孙明瑜) ，NINGBing-bing(宁冰冰)，ZHANGWen-meng(张文萌)’ CHENGao．feng(陈高峰)’，MUYong-ping(慕永平)。，ZHANGHua(张 华)。， LIUJia(刘 佳)，BianYan—qin(边艳琴)，andLIUPing(刘 平)I。 ABSTRACT Objective：ToinvestigatetheeffectsofancientChinesemedicaIformulaXiayuxueDecoction (下瘀血汤，XYXD)onactivationofhepaticstellatecells(HSCs)anddefenestrationofsinusoidalendotheliaI cells(SECs)inCCI4-inducedfibroticIiverofmice．Methods：Highperformanceliquidchromatographywas usedto identifythemaincomponentsofXYXD andcontroIthequalityofextraction．C57BL／6micewere lnducedIiverfibrosisbyCCkexposureandadministeredwithXYXDfor6weekssimultaneously．Livertissue wasinvestigatedbyhematoxylin．eosinandSirius-redstaining．Sinusoidalfenestrationswereobservedby scanningelectronicmicroscopyandfluorescentimmunohistochemistryofPECAM-1(CD31)．WholeIiver lysatesweredetectedof —smoothmuscleactin( ·SMA)andtype-IcollagenbyWesternblot．Primaryrat HSCs-T6cellswereanalyzedbydetecting 0【-SMA，F-actin，DNAfragmentationthroughconfocalmicroscopy， Westernblot，terminal-deoxynucleoitidyltransferasemediatednickendlabeling(TUNEL)assayandcellomics arrayscan，respectively．Results：AmygdalinandemodininXYXDwereidentified．XYXD(993mg／kg)inhibited Siriusredpositiveareaupto70．1％ (P<0．01)，aswellasproteinlevelsof ·SMAandtype—Icollagenby 42．0％and18．5％ (P<0．05)respectively．Invitro，XYXD(12．5 g／mL，50JI,g／mL)suppressedtheactivationof HSCsandreversedthemyofibroblasticHSCsintoquiescent．demonstratedasInhibitionoffluorescentF—actin by32．3％and46．6％ (P<0．05)．Besides，XYXDinducedtheapoptosisofHSC-T6cellsby20．O％ (P<O．05) and49。5