芒属种质资源花粉育性的评价.docVIP

芒属种质资源花粉育性的评价 摘 要：染色法测定花粉活力是一种较为直观、简单、初步的方法，能给育种工作提供最基础的花粉存活信息，从不育或可育花粉的数量上区别不育和可育的程度。花粉是否具有活性，能否被染色，可直观反映雄配子的发育以及花粉的可育情况。本课题以湖南农业大学芒属 ... <p>摘&nbsp; 要：染色法测定花粉活力是一种较为直观、简单、初步的方法，能给育种工作提供最基础的花粉存活信息，从不育或可育花粉的数量上区别不育和可育的程度。花粉是否具有活性，能否被染色，可直观反映雄配子的发育以及花粉的可育情况。本课题以湖南农业大学芒属种质资源圃内来自于全国各地的种质资源作为研究对象。首先跟踪记录某一植株的日开花数，找出日开花高峰期。对资源圃内所有材料的花期进行记录，归纳出不同品种间花期的差异。离体萌发法测定出花粉的萌发力，再用I2-KI染色法测定花粉的活性。萌发力与花粉活性结合分析，可较为全面的对花粉育性做出评价研究。<br /> 关键词：芒属种植；花粉育性；萌发力；活力；评价 <br /> <br /> Pollen Fertility Evaluation Of Miscanthus Germplasm<br /> Abstract: Dyeing law to determine the pollen energy is a more intuitive and simple, elementary method, can provide the basic information to breeding of the work of the pollen live, from sterile or fertile pollen sterile on the difference between quantity and the fertile levels. Whether the pollen is active or will be used in dyeing can directly reflect the development of the male gamete and the fertile pollen.The subject of the Agricultural University, This subject is use the germinal in the Hunan Agricultural University from all parts of china as a study. First of all, record the number of flowers into the sea to find the peak. To record all the material in the garden and then summarized the differences in different varieties flowering. From the body of law to determine the quality of pollen quality, then use the I2-KI, dyeing the determination of activity. Combined the germination ability and the analysis of the pollen can be more comprehensive to pollen sterile sexual evaluate study. <p class='Inc347'></p> <br /> Key words:miscanthus cultivation；pollen fertility ；germination capacity；viability； evaluation <br /> <br /> 2&nbsp; 实验材料与方法&nbsp; <br /> 2.1&nbsp; 实验材料<br /> 本实验所用材料为来自与全国各地的芒属种植资源，包括芒、五节芒、荻、南荻四种。所有材料均由湖南农业大学芒属植物资源圃提供。<br /> 2.2&nbsp; 实验方法<br /> 2.2.1&nbsp; 芒属植物日开花动态测定<br /> 在芒属植物资源圃中选取某一单花序（A0445），从第一朵花开始开花就进行跟踪测定直到开花完毕，进行72小时开花数目跟测。每隔2小时进行一次测定，并通过测定结果得出日开花动态变化情况。<br /> &nbsp; </p> <br /> <br /> <p><br /> 目&nbsp;&nbsp; 录&nbsp;&nbsp; 字<br /> 摘&n