芒属植物转化受体系统的建立.docVIP

芒属植物转化受体系统的建立 摘 要：随着石油、煤炭和天然气等化石燃料的日趋枯竭以及生态环境的不断恶化，开发新的洁净可再生能源已成为全世界关注的战略重点。生物质能它是以化学能形式贮存于生物质中的一种能量，它直接或间接地来源于植物的光合作用，主要为植物能源。芒属植物（Misc ... <p>摘&nbsp; 要：随着石油、煤炭和天然气等化石燃料的日趋枯竭以及生态环境的不断恶化，开发新的洁净可再生能源已成为全世界关注的战略重点。生物质能它是以化学能形式贮存于生物质中的一种能量，它直接或间接地来源于植物的光合作用，主要为植物能源。芒属植物（Miscanthus.）作为一种有潜力的能源植物受到了巨大了关注；同时，它还有改善环境与利用等其他的作用。为了进一步开发芒属植物，获得高产，抗逆性高，纤维含量高的新种质，建立外源基因转化体系，转化受体系统是其基础。本实验对芒属植物的种子、幼穗、芽等外植体在加入了2，4-D、6-BA的MS、NB、CC培养基上分别进行组织培养，筛选出最佳的培养基配方，提高外植体的诱导出愈率，建立芒属植物转化受体系统。 <br /> 关键词：芒属植物；组织培养；转化受体系统。<br /> <br /> Establishment Of Transformation And Receptor System Of Miscanthus.<br /> Abstract: With oil, coal and natural gas and other fossil fuels are depleting and deteriorating ecological environment, the development of new clean renewable energy has become the strategic focus of worldwide concern. It is a form of biomass energy to chemical energy stored in a biomass energy, which directly or indirectly, from plant photosynthesis, mainly for plant energy. Miscanthus. as a potential energy plants has been a great concern; the same time, it also improve the environment and the use of other role. In order to further the development of Miscanthus., high yield, high resistance, high fiber content of new germplasm, the establishment of the foreign gene transformation system is the basis for Transformation System. The experiments on the seeds of Miscanthus., immature inflorescence, shoot substandard implants added 2,4-D ,6-BA of MS, NB, CC tissue culture medium, respectively, selected the best medium formula to improve the explants callus induction rate, the establishment of Miscanthus. Transformation and Receptor System. <br /> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; 2.1.2 实验器材及培养设备5<br /> 2.2 试验方法5<br /> &nbsp;&nbsp;&nbsp; 2.2.1 外植体的培养5<br /> &nbsp;&nbsp;&nbsp; 2.2.1.1 培养基及培养条件5<br /> &nbsp;&nbsp;&nbsp; 2.2.1.1 试验过程5 <span class='Ubq780'></span> <br /> 2.3 数据统计及处理6<br /> 3 实验结果与分析6<br /> 3.1种子为外植体诱导愈伤及分化增殖6<br /> 3.1.1基本培养基和2，4-D不同浓度对愈伤组织诱导的影响6<br /> 3.1.2芽分化及增殖培养7<br />