骨髓基质干细胞凝胶复合体加关节止痛胶囊修复兔膝软骨缺损的实验探究.pdf

提 要 目的：探讨关节止痛胶囊对骨髓基质干细胞凝胶复合体修复兔膝关节软骨缺损的 影响。方法：采用密度梯度离心+贴壁培养法分离、纯化和扩增BMSCs，取第三代BMSCs 制备纤维蛋白凝胶复合体。实验将 40 只新西兰兔，随机分为四组：I 组缺损不做任 何处理；II 组缺损中植入未加细胞因子的凝胶复合体；III 组和 IV 组缺损种植入含 细胞因子的凝胶复合体，II组和IV组每天关节止痛胶囊灌胃。分别于术后第4、8、 12周后取材，进行大体及组织学观察。结果：BMSCs增殖速度快，易扩增可获取足够 数量的细胞来修复软骨缺损。术后12周, IV组缺损完全由透明软骨组织填充，软骨 细胞排列规则，新生软骨的厚度与正常软骨厚度基本相同。结论：骨髓基质干细胞凝 胶复合体加关节止痛胶囊能修复关节软骨缺损，关节止痛胶囊在体内可以诱导骨髓基 质干细胞向软骨细胞分化，促进凝胶复合体修复软骨缺损。 关键词 关节止痛胶囊；骨髓基质干细胞；纤维蛋白凝胶；关节软骨缺损；实验 研究 Experimental Study on Repairing Rabbit Articular Cartilage Defects By Bone Marrow Stromal Cells Gel Complex And Guanjiezhitong Capsule Speciality:Orthopaedics of TCM Author:Zhang Zhaoguo Tutor:Bi Rongxiu Abstract Objective：To explore the effect of Guanjiezhitong capsule and stem cell gel complex on the repair of rabbit articular cartilage defect.Methods ：BMSCs were isolated ， purificated and raised from bone marrow by density gradient centrifuge and adherence culture method.BMSCs at passage 3 were selected to composite with fibrin gel.In the experiment study,40 New Zealand white rabbits altogether 80 sides knee joints,were randomly divided into four groups: Group I implanted without any complex into the defects. Group II implanted with stem cell gel complex without cytokines.Group III and Group IV implanted with stem cell gel complex with cytokines TGF- βand IGF-I. The rabbits inGroup II and Group IV were gavaged Guanjiezhitong capsules daily. The rabbits were sacrificed at 4 ，8，12 weeks.Each specimen was evaluated by both morphology and histology.Results ：BMSCs raise so fast that we can obtain enough cells to repair the defect of articular cartilage. At 12 weeks after