[分子数量遗传学课件]leture5 Comparative mapping.pptVIP

Comparative Positional Candidate gene mapping Reason Study of evolution Prediction of genes from “map rich” species to “map poor” species mouse, human, livestock Rice maize,wheat Real mapping and virtual mapping Definitions Synteny: on the same chromosome. Conservation of synteny: genes found together on one chromosome in species A are also found together in species B. Comparative Mapping Approaches Use Heterologous bidirectional painting (ZOO-FISH). A highly efficient way of generating comparative cytogenetic maps between distantly related mammalian species Bidirectional painting between SSC12 and HSA 17 These two slides demonstrate the interest of bidirectional heterologous hybridisation to establish segment-to-segment matches between porcine and human chromosomes The porcine chromosome SSC 12 used as probe (PARM-PCR) on human metaphase, gave an hybridisation signal on the entire HSA 17 chromosome and no signal on other chromosomes (Fig. a). The reverse hybridisation using HSA 17 as probe (DOP-PCR, Cambio) on porcine chromosomes gave a signal only on the entire SSC 12 (Fig. b). So we conclude that there is correspondence between these two chromosomes over their entire length as reported Comparative Mapping Approaches Use sequence information across species to identify genes. synteny mapping in somatic cell hybrids linkage mapping in situ hybridization radiation hybrid mapping Use ESTs ZOO-Chips for expression studies. Blast BLAST 是由美国国立生物技术信息中心（NCBI）开发的一个基于序列相似性的数据库搜索程序。 BLAST是“局部相似性基本查询工具” (Basic Local Alignment Search Tool)的 缩写。 主要的blast程序 Science, 258:1248-1251 Background Comparative mapping-RN BAC contigs Screened a porcine Bacterial Artificial Chromosome (BAC) library and constructed a 2.5 megabase pair (Mbp) contig of the RN region Linkage map The BAC clones were used to devedevelop new genetic markers in the form of microsatellites(MS) and single nucleotide polymorphisms(SNPs). The markers were used to construct a