基础研究染料木黄酮通过上调血红素氧合酶-1表达减缓野百合碱.pdfVIP

2012;32(2) 南方医科大学学报（J South Med Univ ） ·151 · 基础研究 染料木黄酮通过上调血红素氧合酶-1表达减缓野百合碱诱导的 大鼠肺动脉高压 张玉坤，王导新，朱 涛，李长毅 重庆医科大学附属第二医院呼吸内科，重庆 400010 摘要：目的研究染料木黄酮对野百合碱诱导的肺动脉高压大鼠肺组织血红素氧合酶-1（HO- 1）表达的影响，探讨其减缓肺动脉 高压的机制。方法60 只雄性SD大鼠随机分成对照组、野百合碱组、小剂量染料木黄酮治疗组和大剂量染料木黄酮治疗组。监 测血流动力学变化，通过电子显微镜观察肺小动脉结构重塑，Western blotting 检测肺组织HO- 1 的表达。结果染料木黄酮治疗 组较野百合碱组大鼠平均肺动脉压降低（P0.01），右心肥厚指数下调（P0.01），大剂量组更显著（P0.01）。染料木黄酮治疗组 较野百合碱组大鼠肺组织HO- 1表达上调（P0.01），大剂量组更显著（P0.01）。结论染料木黄酮能减缓野百合碱诱导的大鼠肺 动脉高压，可能与其上调大鼠肺组织HO- 1表达有关。 关键词：肺动脉高压；野百合碱；染料木黄酮；血红素氧和酶-1 中图分类号：R322.121; R563.9; R543.2 文献标志码：A 文章编号：1673-4254（2012）02-0150-04 DOI: CNKI:44-1627/R1526.032 /kcms/detail/44.1627.R1526.032.html Genistein attenuates monocrotaline-induced pulmonary arterial hypertension in rats by up-regulating heme oxygenase-1 expression ZHANG Yukun, WANG Daoxin, ZHU Tao, LI Changyi Department of Respiratory Medicine, Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China Abstract: Objective To study the effect of genistein on the expression of heme oxygenase-1 (HO-1) in rats with pulmonary arterial hypertension (PAH) induced by monocrotaline (MCT). Methods Sixty male Sprague-Dawley rats were randomly divided into 4 groups (n=15), namely the control group, model group, low-dose (20 μg/kg) genistein group and high-dose (80 μg/kg) genistein group. The hemodynamic parameters were measured and the remodeling of pulmonary small arteries was observed by electron microscope (EM). The expression of HO-1 in the lung tissues were detected by Western blotting. Results Compared with the model group, genistein treatment significantly reduced the elevated mean pulmonary arterial pressure, improved the right ventricular hypertrophy index, and increased the expression of HO-1 i