猪胰岛细胞分离纯化研究进展.pdfVIP

1012 Chinese Journal of Reparative and Reconstructive Surgery, August 2012, Vol. 26, No.8 · · • 异体及异种移植• 猪胰岛细胞分离纯化研究进展 朱海涛 于良 王博 【摘  要】    目的 综述猪胰岛细胞分离纯化的常用方法及最新研究进展。 方法 查阅近年来国内外关于猪胰岛 细胞分离纯化的相关文献，进行总结分析。 结果 分离纯化的效果主要取决于供体的前期筛选、高质量胰腺的获取与 有效保存，以及分离纯化方法的选择与改进。 结论 猪胰岛分离纯化操作的优化与标准化的建立，极大促进了异种胰 岛移植研究的发展，有望解决目前移植供体短缺的问题。 【关键词】 胰岛细胞 分离 纯化 异种移植 猪 PROGRESS IN ISOLATION AND PURIFICATION OF PORCINE ISLETS/ZHU Haitao, YU Liang, WANG Bo. Department of Hepatobiliary, the First Affiliated Hospital, Medical School of Xi’an Jiaotong University, Xi’an Shaanxi, 710061, P.R.China. Corresponding author: WANG Bo, E-mail: bobwang75@ 【Abstract 】    Objective To review the common methods of isolation and purification of porcine islets and research progress. Methods Domestic and abroad l iterature concerning the isolation and purification of porcine islets was reviewed and analyzed thoroughly. Results The efficacy of the isolation and purification depends on the selection of donor, the procurement and cryopreservation of high-qual ity donor pancreas, and the selection and improvement of the operation. Conclusion The shortage of transplanted islets could be resolved by the establ ishment of standardized and optimal process, which may also promote the development of porcine islet xenograft. 【Key words 】 Islets Isolation Purification Xenograft    Porcine Foundation item: National Natural Science Foundation of China 胰岛细胞移植是目前治疗 1 型糖尿病较为理想的 胎猪与新生猪胰腺所含的外分泌组织较少，一般 方法，但由于人类同种胰岛供体来源缺乏，限制了其临 采用剪碎加酶消化胰腺组织法，很容易获得纯度较高 床应用，猪胰岛作为理想的异种供体来源日益受到重 的ICCs 。但ICCs 属于未成熟细胞，体内移植实验表 视[1-3] 。如何有效分离并纯化可供移植的功能性猪胰 明[5-6] ，胎猪ICCs 通