薏苡仁油诱导乳腺癌细胞系mcf-7 细胞的凋亡及原理研究.pdfVIP

2007 11 2 Journal of Clinical M edicine in Prac ice # 1 # MCFO7 1, 2 2 2 曹国春 , 梁 军 , 侯亚义 ( 1. 2 10009; 2. , , 2 10093) : M CFO7 M CFO7 , 24 h , M T T ; ( P I) ; 123( Rodamine 123) 530 nm , , M C FO7 ; PI , M CFO7 , ; , 123 M CF7 ; ; , M CFO7 , M CFO7 M CFO7 , : ; M CFO7 ; M T T ; ( P I) ; 123; : R 73719 : A : 1672O2353( 2007) 02O000 1O03 Coix Lacrymajobi L VarmaOyuen Lipid Induces Apoptosis in MCFO7 Cell Line CAO GuoOchun, LIANG un, HOU YaOyi ( 1. Jiangsu Pr ov incial Tumor H osp ital, N anj ing 2 10009; 2. M edical College of N anj ing University 210093 ABSTRACT: In his research w e s udied he ef fec s of coix lacrym ajobi L varmaOyuen lipid on M CFO7 cells, w hich w ere ob ained from human breas adenocarcinoma. Af er being rea ed w i h dif- f eren concen ra ions for 24 hr, he viabili y of MCFO7 cells w as de ec ed by M T T ; apop osis was de ermined by FCM af er being s ained w i h propidium iodine ( P I) , and he membrane po en ial of mi ochondria w as de ec ed by f luorescence in ensi y af er being s ained w i h Rodamine 123. T he re- sul s show ed ha af er being coOcul ured for 24 h, he viabili y of M CFO7 cells had a dow nOregula ed endency wi h he increase of concen ra ions; he apop osis percen ag e in high dose class was more signif ican han ha in lowerOdose rea ed classes; he mi ochondrial membrane po en ial collapsed af er being rea ed w i h coix lacrymajobi L varmaOyuen lipid