改良鸡胚尿囊膜肿瘤实验动物模型及其生物学行为观察综合分析说明.docVIP

  改良鸡胚尿囊膜肿瘤实验动物模型及其生 物学行为观察 马继伟1，张勇1，张逸铠1，马征来2，闫雍容1，杜展1，王超1，夏潮涌2， 5 10 15 20 25 30 35 40 钟雪云1** （1. 暨南大学医学院病理教研室，广州 510632； 2. 暨南大学医学院组织学与胚胎学教研室，广州 510632） 摘要：目的：利用鸡胚尿囊膜建立人移植瘤模型并观察其生物学行为和组织学形态已有报道。 但是，在实际工作的过程中仍存在不少问题。因此，有必要通过改良的方法，获得简单，快 捷，可重复的动物模型。方法：将人胶质瘤 SWO-38 细胞接种至鸡胚尿囊膜表面，观察影响 鸡胚的存活和成瘤的可能因素、移植瘤的生长特性和形态学特征。结果：利用改良的方法成 功建立胶质瘤鸡胚尿囊膜的模型；肿瘤组织能够诱导血管生成，镜下形态符合恶性胶质瘤的 形态学特征。结论：该模型简单，快捷，易于建立。便于观察肿瘤组织的生物学行为，可用 于抗肿瘤的药物筛选。 关键词：胶质瘤；鸡胚尿囊膜；移植瘤；血管生成 中图分类号：R739.4 Improvement and biological behavior observation of human xenograft model on chick embryo chorioallantoic membrane MA Jiwei1, ZHANG Yong1, ZHANG Yikai1, MA Zhenglai2, YAN Yongrong1, DU Zhan1, WANG Chao1, XIA Chaoyong2, ZHONG Xueyun1 (1. Department of pathology, medical school, Jinan University, GuangZhou 510632; 2. Department of histology and embryology, medical school, Jinan University, GuangZhou 510632) Abstract: Objective : Establising human xenograft model on the chick embryo chorioallantoic membrane (CAM) and observing its biological behavior have been reported , however ,problems remain , In order to obtain a model which is easy to be duplicated, improving some technique is necessary. Methods : Human glioma cell line SWO-38 was inoculated on the chick embryo chorioallantoic membrane to observe the factors affecting the survival of the transplanted human glioma cells, growth characteristics of the transplanting tumor and the morphological feature. Result : Human glioma model was successfully established using the modified method it shows angiogenesis effects and morphological characteristics of glioma cells. Conclusions：This model can be easily duplicated, used in biological behavior observation and the research of selection for antineoplastic drugs. Keywords: Glioma; Chick embryo chorioallantoic membrane (CAM); Xenograft model; Angionesis 0 引言 动物模型是研究疾病发生发展及相关机制的重要手段，目前常用的体内实验模型为裸 鼠，而裸鼠模型因其实验周期长，费用昂贵、实验条件要求高，限制了它的广泛应用。近年 来,鸡胚尿囊膜（chick choriollantoic membrane,CAM）模型在肿瘤血管方面的研究受到关注。 因鸡胚发育的 9-14 天，免疫系统并没有完全形成,排斥反应尚