Assessment of inner filter effect corrections in fluorimetry教程.pdfVIP

Freseniu$ Journal of Fresenius J Anal Chem (1991) 340:341 -344 @ Springer-Verlag1991 Assessment of inner filter effect corrections in fluorimetry M. M. Puchalski 1 M. J. Morra 1, and R. yon Wandruszka 2 1 Division of Soil Science, University of Idaho, Moscow, ID 83843, USA 2 Department of Chemistry, University of Idaho, Moscow, ID 83843, USA Received February 6, 1991 Summary. The inner filter effect (IFE) in fluorescence in a decreased fluorescence intensity, it is not a quenching spectroscopy is not easily distinguished from dynamic and effect [2]. In order to accurately determine Stern-Volmer static quenching phenomena, since IFE rarely occurs quenching constants, IFE must be distinguished from true without quenching. IFE corrections may be subject to under- quenching. Several methods have been proposed to correct or over-compensation effects that are difficult to assess accu- for IFE [1 - 9], but no practical comparison of these meth- rately. To evaluate existing IFE correction procedures, it is ods has been reported. Simple comparison between corrected proposed that the linearity of resulting Stern-Volmer plots fluorescence values and those obtained before addition of and the relative change of their slopes with temperature be the IFE compound does not provide an accurate assessment adopted as criteria. Three correction methods are assayed in of the adequacy of correctio