八肋游仆虫两类释放因子的相互作用.pdfVIP

Acta Genetica Sinica, May 2004, 31 ( 5) : 460~ 467 ISSN 0379- 4172 Interaction of Two Classes of Release Factors from Euplotes octocarinatus 1, 2 1 1 1 1, CHAI BaoF ng , SONG Li , FU Yu Jun , WANG W i , LIANG AiHua ( 1Key L aboratory of Chemical Biology and Molecular Engineering of Education Ministry , Shanxi Uni ersity , Taiyuan 030006, China; 2Institute of Hydrobiology , Chinese A cademy of Sciences , Wuhan 430072, China) Abstract: Translation t rmination on th ribosom is an ss ntial proc ss for c ll viability .This proc ss is maintain d by two class s of p ptid r l as factors ( RF1/ RF2, RF3 and RF1, RF3 in prokaryot s and ukaryot s, r sp ctiv ly) . In protozoa ciliat s Eu p lotes octocarinatus, an unic llular ukaryot s, univ rsal stop codon UGA is r assign d for cyst in sugg sting th sp cificity of vo lution of translation t rmination syst m. W clon d two class s of r l as factors from Eup lotes octocarinatus pr viously. In this pa p r, thr infram stop codons UGA in EoeRF3 g n w r mutat d m diat d by PCR sit dir ct d mutag n sis m thod.Th int r action b tw n RF1 and RF3 from E. octocarinatus was assay d in i o using Y ast Twohybrid Syst m, which has an advantag of highly s nsitivity.Th r sults show d that th RF1 RF3 compl x was form d in living c lls to function in th proc ss of trans lation t rmination, diff ring from that in prokaryot s in which RF1/ RF2 and RF3 function s parat ly. Th volution of translation t rmination of lif form was analyz d using phylog n tic tr of amino acids s qu nc s of RFs ( 32( ) RF1s and 24( ) RF3s) ob tain d from G nBank. Two class s of RFs ar us ful information in analysis of volution of lif form and furth r lucidation of m ch anism of translation t rmination of