基于线粒体序列对泰山赤鳞鱼系统分类和进化的的研究.pdfVIP

Mitochondrial partial DNA sequences analysis of the Chi-lin fish from Tai Mountain: implication for its systematics and evolution Qu Xiaojuan，Zhao Bosheng * ，Wang Yuan School of Life Sciences，Shandong University of Technology，Zibo ，Shandong （255049 ） Email ：zhaobosheng@ Abstract Mitochondrial 12S rRNA, 16S rRNA, cytochrome b, ATP synthase 8 and 6 gene fragments of Chi-lin fish from Tai Mountain were amplified by PCR, the products were purified and sequenced. The sequences and characters of the multiple genes of mitochondrial DNA (mtDNA) were analyzed by MEGA 3.1. The results showed that all of the multiple genes were A-rich; the base compositions of the protein-coding genes were globally G-deficient, especially in the third position. Interestingly, transitions of protein-coding genes were major in the substitution, and largely concentrated in the third position, too. All molecular phylogenetic trees revealed that Chi-lin fish did not belong to genus Varicorhinus, against the former morphological data. And it was comparatively closer to genus Spinibarbus and Barbus . The Cyt b sequence analysis suggested that the divergence time of Chi-lin fish was about 16.0 Mya (million years old) when Tai Mountain was just in Middle Miocene and suffered the aftereffect of Yan Shanian movement. It inferred that the vicariance events were the primary momentum to promote forming the limited distributing of the present. Keywords ：Chi-lin fish ；12S rRNA；16S rRNA；cytochrome b (Cyt b) ；ATP synthase 8/6 (ATPase 8/6)；Phylogeny 1. Introduction About 2500 million years ago, the most age-old eras in the geolog