乳腺癌特异性多肽介导的HSV-TK-GCV系统的构建及其靶向杀伤效应.pdfVIP

网络出版时间：2011-7-14 11:00 网络出版地址：/kcms/detail/31.1725.r1100.001.html http / / www. biother . org Chin J Cancer Biother Aug. 2011Vol. 18No. 4 383 DOI ：10 . 3872 /j . issn . 1007-385X. 2011. 04 . 008 乳腺癌特异性多肽介导的 H SV-TK / GCV 系统的构建及其靶向杀伤效应 650032 ［摘 要 ］ MDA-MB-231 PI HSV-TK/ GCV MDA-MB-231 PCR pORF-HSV-TK PI-TKpET-28a+ pET-28a + -PI-TK IPTG PI-TK His-Tag SDS Western blotting PI-TK PI-TK MDA-MB-231 ganci- clovier GCVCCK-8 MDA-MB-231 pET-28a + -PI-TKPI-TK SDSWestern blotting PI-TK PI-TK MDA-MB-231 PI-TK GCV MDA- MB-231 200 g/ ml PI-TK + 10 mg/ L GCV 68. 9 ±7.57% PI-TK GCV MDA-MB-231 IC50 152 . 64 g/ ml MDA-MB-435 P 0 . 05PI HSV-TK/ GCV MDA-MB-231 ［关键词 ］PI MDA-MB-231 HSV-TK/ GCV ［ 图分类号 ］R734 . 2 R392 . 1 ［文献标志码 ］A ［文章编号 ］1007-385X201104-0383-06 Con str uction and tar geted effect s of br east cancer sp ecific p eptide-mediated H SV-TK / GCV anti-tumor system GENG Ji-weiDONG Jian LIU Wei-qingGAO Chang-eXIONG Qiu-xiaMIAO Yan-dongZHOU Hua-huaLI Qiu- tian LI Chen Biotherapy Center First Affiliated Hospital of Kunming Medical CollegeKunming 650032 Yunnan China ［Ab str act ］Obj ective To investigate the targeted killing effect of breast cancer specific peptide PI-mediated HSV-TK/ GCV system against human breast cancer MDA-MB-231 cells in vitro. Methods PI-TK gene was amplified from pORF- HSV-TK plasmid by PCRand was re −insertedintotheprokaryoticexpressionplasmidpET-28a+ the pET-28a + -PI-TK plasmid was constructed and transfected into the host bacteria. After induction with IPTGPI-TK fusion pro- tein was purified by His-Tag and further identified by SDSand Western blotting anal