NO和K+通道参与乙酰胆碱引起大鼠离体输精管平滑肌细胞超极化.pdfVIP

Acta Physiologica Sinica , June 25, 2007, 59 (3): 331-338 331 Research Paper ACh-evoked membrane hyperpolarization in smooth muscle cells of rat + channels and NO vas deferens in vitro: Involvement of K 1,2 1 1 1,3,* 1,** 1 1 FAN Pin , LI Li , LIU Zheng-Jiang , SI Jun-Qiang , ZHANG Zhi-Qin , ZHAO Lei , MA Ke-Tao 1Laboratory of Xinjiang Endemic and Ethnic Diseases, Institute of Electrophysiology, Medical College Shihezi University, Shihezi 832002, China; 2 3 The First Teaching Hospital of Xinjiang Medical College, Wulumuqi 830054, China; Department of Physiology, Wuhan University School of Medicine, Wuhan 430071, China Abstract: To explore the underlying mechanism of acetylcholine (ACh)-evoked membrane hyperpolarizing response in isolated rat vas deferens smooth muscle cells (SMCs), intracellular microelectrode recording technique and intracellular microelectrophoresis fluorescent staining technique were used to study ACh-evoked membrane hyperpolarizing response in SMCs freshly isolated from Wistar rat vas deferens. By using microelectrodes containing fluorescent dye 0.1% propidium iodide (PI), 37 and 17 cells were identified as SMCs in outer longitudinal and inner circular muscular layers, respectively. The resting membrane potentials of SMCs were (–53.56±3.88) mV and (–51.62±4.27) mV, respectively. The membrane input resistances were (2 245.60±372.50) MΩ and (2 101.50±513.50) MΩ, respectively. ACh evoked membrane hyperpolarizing response in a concentration-dependent manner with an EC50 of 36 µmol/L. This action of ACh was abolished by both a non-sepcific muscarinic (M) re