尼罗罗非鱼两种GHR克隆、组织分布及在不同性腺发育阶段表达变化.pdfVIP

Two growth hormone receptors in Nile tilapia (Oreochromis niloticus): molecular characterization, tissue distribution and expression profiles in gonad during reproductive cycle 1 1 * Xilan Ma , Xiaochun Liu , Yong Zhang, Haoran Lin Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, PR China 1These two authors contributed equally to this work. *Corresponding author: E-mail:ls32@ 1 Abstract Two cDNAs encoding growth hormone receptor (GHR) were isolated from Nile tilapia (Oreochromis niloticus) liver tissue. The two cDNAs, one consisting of 1 908 bp and the other of 1 728 bp, encoding for putative 635- and 575-amino acid GHR (designated ntGHR1 and ntGHR2, respectively), shared 34.4% identity in nucleotide sequence and 29.6% in deduced amino acid sequence. ntGHR1 and ntGHR2 showed conserved structural characteristics of GHR, including the FGEFS motif, the box1 and box2 regions, and extracellular cysteine residues and intracellular tyrosine residues. On the other hand, ntGHR1 and ntGHR2 showed distinctly different features in structure, ntGHR2 lacked one pair of extracellular cysteines (C5, C6) and 4 intracellular tyrosine residues (Y1, Y3, Y6, and Y7) which were conservative in GHR1. Northern blot analysis indicated a single 6.0-kb transcript of ntGHR1 and a single 4.0-kb tran