应用高效液相色谱法及薄层层析法对真菌融合菌株发酵液的鉴别.pdfVIP

理化检验 - 化学分册　　　　P TCA ( PA R T B :C H EM . AN AL . ) 　　　　2009 年 第 45 卷 2 工作简报 应用高效液相色谱法及薄层层析法 对真菌融合菌株发酵液的鉴别 1 1 2 刘 　玲 , 刘长江 , 叶　博 ( 1. 沈阳农业大学 食品学院 , 沈阳 110 16 1 ; 　2 . 辽宁名特优农产品开发服务中心 , 沈阳 110 16 1) 摘 　要 : 为确定两株真菌形成的融合菌株的有效性 , 以融合菌株和双亲本发酵液为研究对象 ,进 行提取 、离心、浓缩等预处理后 ,采用高效液相色谱法及薄层层析法对真菌融合菌株发酵液成分进 行分析 。对比融合菌株与两株真菌亲本因降解能力的不同而使发酵液组分产生的差异 ,从而鉴别 融合菌株形成的真伪 。 关键词 : 高效液相色谱法 ; 薄层色谱法 ; 融合菌株 ; 鉴别 中图分类号 : O652 . 63 　　　文献标志码 : A 　　　文章编号 : 100 14020 (2009) 020 13803 Diff erentiation of Fermentation L iquors of Fungus Crasis by HPL C and TL C 1 1 2 L IU L ing , L IU Changj iang , Y E Bo ( 1. Col leg e of Food S cience , S heny ang A g ricul t u ral Uni ve rs i ty , S hengy ang 110 16 1 , Chi na; ) 2 . L i aoni ng S e rv ice Center of D evelop ment of Ex cel lent A g ricul t u ral P rod ucts , S heny ang 110 16 1 , Chi na Abstract : Samp les of ferment ation liquor s of fungu s car si s and t heir p arent s were t aken for t he st udy . The samp le liquor s were p ret reat ed accor ding to p rescribed p rocedure including ext raction , cent rifuging , concent ration and etc . An aliquot of t he concent rat ed samp le solution wa s u sed for H PL C and TL C det ermination s. It was found t hat due to t he difference of degradation abilit y of fungu s cra si s and t heir p arant s in t he ferment ed liquor s , different degradation p roduct s were obt ained in different samp le liquor s , on t he base of which differentiation of fungu s