百部二元指纹图谱建立及在百部质量评价中应用与研究.docVIP

百部二元指纹图谱的建立及其在百部质量评价中的应用研究 樊兰兰1 ，胡君萍1,2, 倪斯然1, 陈虎彪1,3，蔡少青1﹡（1．北京大学药学院天然药物学系，北京100191；2．新疆医科大学药学院，乌鲁木齐, 830054;3. 香港浸会大学中医药学院，香港） 摘要:目的　采用高效液相色谱法研究并建立百部的二元指纹图谱I和碱性色谱图-指纹图谱II), 考察了地下部分的不同部位、不同加工方法对百部化学成分的影响, 进而比较药典收载三种。方法　色谱柱Merck Purospher STAR RP-18 (4.6mm i.d.×250mm，5μm),I流动相为0.01%磷酸(m/v)和乙腈,II流动相为0.05%氨水(v/v)和乙腈。检测波长：210nm；柱温：25℃；流速：1.0 ml/min。。。结果该方法。。结论二元指纹图谱为科学评价及有效控制药材的质量提供依据。 关键词:;二元指纹图谱 Binary chromatographic fingerprinting analysis of Stemonae Radix from Chinese pharmacopeia and its application for quality control Fan Lan-Lan1, Hu Jun-Ping2, Ni Si-Ran1, CHEN Hu-Biao1,3 , CAI Shao-Qing1*(1. School of Pharmaceutical Sciences, Peking University Health Science Center, Beijing, 100191, China; 2. School of Pharmaceutical Sciences, Xinjiang Medical University, Urumqi, 830054; 3. School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China) ABSTRACT: OBJECTIVE　A binary chromatographic fingerprint method fingerprint I and a basic chromatogram- fingerprint II) was developed to find out the variances among different locations of the underground part of Stemona plants, among different producing process, and to demonstrate overall chemical characterization of the three authoritative origins of Stemonae Radix. METHODS　A HPLC analysis was carried out on a Merck Purospher STAR RP-18 (4.6mm i.d.×250mm，5μm) with the mobile phase ingerprint I containing solvent A (0.01% H3PO4, m/v) and solvent B (CH3CN), while fingerprint II including solvent A (0.05% ammonia, v/v) and solvent B (CH3CN) in gradient mode. The detection wavelength was set at 210 nm, the column temperature was kept at 25 ℃ and the flow rate were 1.0 mL min?. 0Fingerprints of different locations of the underground part and different producing process were compared. Thirty-five samples were analyzed including 8 Stemona sessilifolia (SS), 6 S. japonica (SJ), 19 S. tuberosa (ST), 1 S. parviflora (SP) and 1counterfeit Asparagus species (AF). 20 major compounds were chosen as the chemical makers for Prin