Research Industrialization of Mutation Breeding of Streptomyces Avermitilis Irradiated by Heavy Ion Beam and Fermentation Pilot Production Line Established.pdfVIP

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Research Industrialization of Mutation Breeding of Streptomyces Avermitilis Irradiated by Heavy Ion Beam and Fermentation Pilot Production Line Established.pdf

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Research Industrialization of Mutation Breeding of Streptomyces Avermitilis Irradiated by Heavy Ion Beam and Fermentation Pilot Production Line Established.pdf

126 IMP HIRFLAnnualReport 2009 thirdgenerationinHQ06，butinHQ26thecontentofGABA inthethirdgenerationwasdecreasedsignifi— cantlythanthatin thesecondgeneration．SoweconcludethatHQ06isastablehighGABA—producing strain，wellHQ26needfurtherselectionandsubculture．Inconclusion，ourresultsshowedthatitisfeasi— bleforbreeding high GABA producing strainswith heavy ion beam radiation treatment．The heavy ion beam isakindofeffectivemutationsource． References [1]chenYing。ShenYan，Yao Huiyuan，MachineryforCerealsOilandFoodProcessing inChinese ，4 2005 8Z． [2]JiangDonghua，HuangDanian。RenBufan，eta1．，IndustrialMicrobiology inChinese ，37，2 2007 7． [3]JiangDonghua，HouJiaheng，LiJie，eta1．，JournalofZhejiangNormalUniversity Nat．Sci． inChinese ，3O，4 2007 447． [43XuJianjun，JiangBo，XuShiying．FoodScienceandTechnology inChinese ，10 2002 7． Es]LiuQing，YaoHuiyuan，ZhangHui．AminoAcids BioticResources inChinese ，26，1 2004 4O． 3-·37 Research Industrialization ofM utation Breedingof StreptomycesAvermitilisIrradiated byHeavy IonBeam andFermentation PilotProduction LineEstablished WangShuyang，ChenJihong，LiWenjiang，LiuJing，BoYonghengandCaoYunfei “Researchand industrialization on theM utation Breeding ofStreptomycesAverm itilisirradiated by heavyIonBeam ”。hasmadeaphaseprogressthroughcooperativeeffortsofallparties． Eightstrainswhichpotencywas5300~／g／mlwassieved．afterthemutagenesisprocessingbyheavy ionbeam andthescreeningoforthomutationstrains ． Stabilityofhigh—yieldingstrainswasstudied．Thebasicgrowth characteristicsofStreptomycesaver— mitiliswasgraspedandhaspreferablysolvedtheproblem ofdegradedstrainthroughthestrainseparation， purification，screeningandpassage；aftertheoptimizationoffermentationconditions，thefermentationti— terstabilizedata4500 “g／mlaroundc． Fig． 1 RTY—MZIOOL一1000L Fermentation Pilot Fig．2The5tontankspilotproduction lin