基因表达谱芯片筛查子宫内膜癌相关基因的研究.pdfVIP

288 黄曙方, 李小毛, 李 田 应用基因芯片技术研究子宫内膜癌的基因表达谱, 探讨子宫内膜癌的发生发展 肿瘤相关的 基因群及其功能 应用 TR ZOL 一步法抽提 11例子宫内膜癌患者的子宫内膜和 3例子宫肌瘤患者的正常增殖期 子宫内膜组织的总RNA, 并纯化mRNA, 将等量的子宫内膜的mRNA 反转录合成以Cy5和 Cy3标记的 cDNA链做探针, 混合后在含有 8 064条双点人类全长基因的芯片上进行杂交, 再经芯片扫描图像分析处理后, 分析子宫内膜癌组织 及其 正常增殖期子宫内膜基因表达谱的差异 11例子宫内膜癌组织和 3例正常增殖期子宫内膜组织比较, 含 8 064 个基因的基因芯片中共筛选出差异基因 274条, 其中上调 92条, 下调 182 条 子宫内膜癌的子宫内膜组 织和正常增殖期子宫内膜组织的基因表达谱存在差异, 这些差异基因涉及细胞外基质调节基因细胞因子促癌基因/ 抑癌基因细胞周期细胞凋亡等, 提示这些基因 子宫内膜癌的发生和发展有关 寡核苷酸序列分析; 子宫内膜肿瘤; 基因表达谱 R 73733 A 1007- 9572 ( 2009) 02- 0288- 04 A Prelim inary Study on GeneExpressionProfiling ofEndometrialAdenocarcinoma H UANG Shu-f ang, LIX iao-mao, LI Tian.D ep artment of Gynecology and bstetrics, Guangdong ProvincialPeop lesH osp ita,l Guangzhou 5 10080, China Abstract Objective T o investigate, us ing gene ch ip technique, the gene expression profiling of endom etrial carc-i nom a, the tum or- assoc iated genogroups in its in itiation and p rogression, and function. Methods T otal RNA w as ex tracted, using a standard TR ZOL protoco,l from 11 endom etrial adenocarc inom a samp les and m atched norm al tissues, and DNA w as la- beled fluorescence during reverse transcription. Exper mi ent group ( carcinom a samp les) were labeled Cy - 5- dCT P and control group ( normal tissues) Cy - 3- dCT P. M icroarray w ith 8 064 genesw as supported by Ch ipscreen B ioscience; equal quantitative probes of both groups werem ix ed and hybr id ized on them icroarray; the Cy5: Cy3 ratiow as obta ined by computer analysis. Re- sults Prelmi inary analysis of gene expression profiling show ed that 274 genes expressed d ifferen tially betw een 11 carcinom a sam- p les and norm al tissues w ere cho sen from 8 064 genes in wh