AO_EB双重荧光染色检测细胞凋亡的研究.pdfVIP

( ) ( ) 　　　　　　　　　　　　　　　　　　　　　　　　　　　　郧阳医学院学报 J 　YMC 　1997 年 6 月 ,16 2 :68 68 AO/ EB 双重荧光染色检测细胞凋亡的研究 1 1 2 1 熊 　琛 　陈绪云 　郭怀兰 　杨 　靖 ( 1 2 ) 郧阳医学院 微生物学教研室 　 预防医学教研室 　十堰 442000 摘 　要 　目的 :检测细胞培养中凋亡细胞的形态及数量。方法 :取 4 ℃环境下不同时间培养的小鼠 胸腺细胞 ,用 AO/ EB 染色后荧光镜观察。结果 :荧光镜下早期凋亡细胞发绿色或暗绿色荧光 ,伴有 核结构的变化 ,晚期凋亡细胞发橙红色荧光并伴有凋亡小体的形成。结论 :AO 和 EB 双重荧光染色 后 ,荧光镜检在细胞凋亡检测中既可定性 ,又可定量。 关键词 　凋亡 　AO 和 EB 双重荧光染色 中图法分类号 　R392. 12 The Use of AO/ EB Double Staining for Detection of Apoptosis Xiong Chen ,Chen Xuyun , Guo Huailan et al ( ) Depart ment of Microbiology , Y unyan g Medical College , S hiyan 442000 Abstract 　Objective : To detect the forms and amount of murine thymocytes through cell cultivation. Methods :Murine thymocytes cultured at a temperature of 4 ℃and stained with AO/ EB were observed under a fluoroscope at different time. Results :Under fiuoro scope ,early apoptosis cells were seen to emit green or dark green fluoroscopes ,with nucle ar morpholo gical changes. Late apoptosis cells ,however ,sent out orange - colored fluores cence ,with formation of apoptosis bodies. Conclusions :Both qualitative and quantitative analysis of the apoptosis can be made with the AO/ EB double fluorescent staining tech nique. Key words 　apoptosis ; AO/ EB double fluorescence staining ( ) 　　凋亡 apoptosis 是多细胞动物生命活动 作用。 过程中一种受控于基因指导的主动性细胞自