JAK2STAT3 通路在肝癌细胞侵袭及血管生成拟态中的作用.docVIP

JAK2/STAT3通路在肝癌细胞侵袭及血管生成拟态中的作用△ 王晔飞1李洪涛1 宋立文1 刘波2 【摘要】目的：探讨JAK2/STAT3通路在肝癌细胞QGY-7701侵袭及血管拟态中的作用，为临床治疗肝癌提供新的治疗靶点。方法：JAK2抑制剂AG490(5μmol/L、10μmol/L)处理肝癌细胞48h，transwell小室、体外成管实验分别检测各组细胞的体外侵袭能力和成管能力，RT-PCR检测Twist1及MMP-2mRNA表达差异，Wetern Blot检测STAT3、p-STAT3、Twist1和MMP-2蛋白表达差异。结果：与对照组相比，transwell小室穿膜细胞数减少，形成管道结构数目减少，Twist1及MMP-2mRNA表达减少，Twist1、MMP-2和p-STAT3蛋白表达减少，差异均有统计学意义（P＜0.05），STAT3蛋白表达无差异（P＞0.05）。结论： AG490能有效抑制肝癌细胞侵袭及成管的能力，JAK2/STAT3通路在肝癌细胞侵袭及血管拟态中起促进作用。 【关键词】 肝癌 AG490 侵袭 血管拟态 【中图法分类号】R735.7 R966 R977.6 The role JAK2/STAT3 signaling pathway played in invation and Vascular Mimicry of liver cancer cell Pang Linbin1, Wang Honglin1△, Wang YeFei1, Li Hongtao1,Song Liwen1,Liu Bo2 [Abstract] Objective To study the role that JAK2/STAT3 signalling pathway played in invation and vascular mimicry of liver cancer cell QGY-7701, provide a new target for clinic treatment of liver cancer. Method Treating liver cancer cell with 5μmol/L and 10μmol/L AG490, the inhibitor of JAK2， for 48h, invating and forming tubes abilities of tumor cells are tested in vitro with transwell and vascular mimicry experiment.RT-PCR to study expression differences of Twist1 and MMP-2 mRNAs;Western Blot to study expression differences of p-STAT3,STAT3,Twist1 and MMP-2 proteins. Result Compared to control group, the number of membrane- permiating tumor cells and tubes formed by tumor cells are reduced; RT-PCR show that the expression level of Twist1 and MMP-2 mRNA are decreased; Wstern Blot show that the expression level of p-STAT3 ,Twist1,MMP-2 proteins are decreased(P0.05),while there are no difference of STAT3 protein among each groups(P0.05).Conclusion The abilities of invation and Vascular Mimicry of liver cancer cells can be inhibited by AG490 in vitro, JAK2/STAT3 signaling pathway promotes the abilities of invation and Vascular Mimicry of liver cancer cells. 【Key Word】 liver cancer AG490 invation Vascular Mimicry 血管拟态是近年来兴起的影响肿瘤转移的学说，逐渐成为研究热点。JAK2/STAT3通路已被证实与多种肿瘤的侵袭转移