药疹病人EB病毒和人疱疹病毒6型感染检测_医学论文.docVIP

药疹病人EB病毒和人疱疹病毒6型感染检测_医学论文 药疹病人EB病毒和人疱疹病毒6型感染检测_医学论文 作者：杨婷婷 朱桂芝 陈官芝 罗兵 【摘要】 目的 探讨EB病毒（EBV）和人疱疹病毒6型（HHV6）感染在药疹发生中的作用。方法 采用PCR检测药疹病人和正常对照组外周血单个核细胞中EBV DNA；采用巢式聚合酶链反应检测HHV6 DNA，并对HHV6阳性标本进行酶切分型；采用ELISA法检测EBV VCA；采用RT检测EBV即刻早期基因 BZLF1 mRNA。结果 药疹病人EBV DNA阳性率(77.42%，24/31)明显高于正常对照组（χ2=77.84，0.01），但HHV6阳性率（32.26%，10/31）与正常对照组比较差异无显著性（χ2=3.00，Pgt0.05）。10例HHV6阳性药疹病人全部为HHV6A 亚型，未检测到HHV6B亚型；而正常对照组73例HHV6阳性标本中有13例为HHV6A亚型。药疹病人EBV VCA阳性率（19.34%，6/31）与正常对照组比较差异具有显著意义(χ2=4.61，0.05）。10例HHV6阳性药疹病人中有9例检出EBV DNA，5例BZLF1 mRNA阳性病人中有4例检出HHV6 DNA，药疹病人BZLF1 mRNA检出率与HHV6有明显的相关性（r=0.45，0.05）。结论 药疹病人存在EBV的活动性感染，且EBV和HHV6可能有相互激活作用。 【关键词】 药疹；疱疹病毒4型,人；疱疹病毒6型,人 ［ABSTRACT］ Objective To study the role of EpsteinBarr virus (EBV) and Human Herpesvirus 6 infection in drugeruption patients. Methods EBV DNA was tested in druperuption patients and normal control by PCRSouthern blotting, and HHV6 DNA by nestedpolymerase chain reation, the positive samples were then genotyped,the EBV VCAIgM was tested by ELISA. The expression of BZLF1 mRNA was tested by RTPCRSouthern in EBV positive samples. Results The positive rate of EBV DNA (24/31,77.42%) in the patients was higher than the normal control (χ2=77.84,0.01),but the difference of HHV6 was not significant (Pgt0.05). In 10 HHV6positive patients, all were HHV6A subtype, no HHV6B subtype was found but in 73 positive cases of the normal control, HHV6A subtype was noted in 13. The positive rate of EBV VCAIgM in the patients was 19.34% (6/31), the difference was significant as compared with the normal control (χ2=4.61,0.05). In 10 HHV6 positive patients, nine were detected to have EBV DNA in five with BZLF1 mRNA positive, four were noted with HHV6 DNA, the detection rate of BZLF1 mRNA was dramatically correlated with HHV6 (r=0.45,0.05). Conclusion There is an active infection of EBV in patients with drug rash, a reciprocal activation may exist EBV and HHV6. ［KEY WORDS］ Drug eruption Herpesvirus 4, human Herpesvirus 6, human 药疹又称为药物性皮炎，轻者仅有皮疹，重者可并发