根癌脓杆菌介导的旱柳生长点转基因的研究.pdfVIP

pines from the wilt disease. In this study, the genome of P. fluorescens GcM5-1A was sequenced using a Roche 454 GLX sequencer. Althogether, over 1.5 million high quality reads are obtained, which comprise of 600Mb oligonucleotides. These sequences are about ca. 100x sequencing depth of the P. fluorescens GcM5-1A genome. Subsequently, the obtained sequences are assembled by the in-house Newbler software. Totally, the assembled sequences cover a physical length about 6.01Mb. Contig N50 is 76kb, and 79 contigs are greater