耐亚胺培南铜绿假单胞菌金属_内酰胺酶基因研究.pdfVIP

2008 18 11 1509 1 1 2 1 赵书平 , 姜梅杰 , 田 鑫 , 郝巧光 ( 1. , 271000; 2. , 271000) : ( PCR) IMPVIMSPMGIM 62 16 IMP 5 VIM , SPM GIM : ; ; ; + : R378. 99 1 : A : 2008) Drug Resistance Genes in Imipenemresistant Pseud omonas aerug inosa * ZHA O Shu ing , JIANG M eijie, T IAN Xin, HA O Qiaoguang ( * Centr al H osp i tal of T ai an, T ai an, Shandong 271000, Chi na) Abstract: OBJECTIVE To study t he drug resistance genes in imi enemresistant Pse udomonas aer ug inos a . METHODS Five main drug resistance genes: IMP, VIM , SPM and GIM which are ert inent w ith metal lactamase and outer membrane rotein o rD gene w ere analyzed using PCR method. RESULTST he o rD genes in 2 2 48 strains of imi enemresist ant P . aer ug inosa w ere negative, and in 14 st rains w ere osit ive from all 62 strains tested. There w ere 16 strains w ith osit ive IMP ty e and 5 strains w ith ositive VIM ty e metal enzyme genes ositive, 7 metal enzymes roducing strains w ere w ith negative o rD2 gene. T he SPM and GIM metal enzyme genes detected w ere all negative. CO CLUSIO S The loss of outer membrane rotein o rD gene is the main 2 mechanism of imi enem resistance in P . aer ug inos a in Tai an area. Key words: Pse udomonas aer ug inosa ; Imi enem; Resistance mechanism; Gene KB 136 , ( PAE) 18 , [ 1, 2] IPM + EDT A CAZ+ EDT A ( IMP) PAE , 2005 10 - 2006 10 1. 3 菌株鉴定及药敏试验 62 ( IRPA) A TB IMPVIM SPM G