急性髓细胞白血病病人TCRζ链基因表达特点.doc

急性髓细胞白血病病人TCRζ链基因表达特点 陈 思， 李扬秋， 陈少华， 杨力建， 吴秀丽， 岑东芝 （暨南大学医学院血液病研究所， 广州 510632） [摘要] 目的 建立实时定量PCR方法检测TCRζ链表达水平的方法，了解急性髓细胞性白血病病人外周血TCRζ链基因表达水平。方法 采用SYBR GreenⅠ荧光定量PCR和相对定量分析法检测31例急性髓细胞性白血病患者（M1型6例，M2型15例, M3型10例）和30例正常人外周血的单个核细胞的TCRζ链表达情况，以β2微球蛋白基因（β2M）作为内参,根据相对定量公式：2－ △△Ct计算急性髓细胞性白血病患者与正常人TCRζ链表达差异倍数。结果 与正常人相比，急性髓细胞性白血病患者TCRζ链表达特点可以分为表达缺失（16%）、表达下降（26%）和表达上升（58%）三种情况。而种不同亚型AML之间的TCRζ链表达模式相似。结论 本研究成功建立SYBR GreenⅠ荧光实时定量PCR和相关定量分析TCRζ链表达水平技术，并首先报道了AML病人TCRζ链表达水平的变化。 [关键词] TCRζ链；实时定量PCR；急性髓细胞性白血病 [中图分类号] R733.7 [文献标识码] A 10.3969/j.issn.1671-5144.2013 The Feature of TCR-zeta Chain Expression in Patients with Acute Myeloid Leukemia CHEN Si，LI Yang-qiu，CHEN Shao-hua，YANG Li-jian，WU Xiu-li，CEN Dong-zhi （Institute of Hematology，Medical College，Jinan University，Guangzhou 510632，China） Abstract: Objective To establish a real-time PCR technique for detection and quantify of TCR ζ chain expression and to investigate TCR ζ chain gene expression level in patients with acute myeloid leukemia (AML)．Methods Real-Time PCR with SYBR GreenⅠtechnique was used for detecting TCR ζ chain expression level in peripheral blood mononuclear cells of 31 patients with AML [including 6 cases with AML-M1 subtype (M1), 15 cases with M2, 10 cases with M3] and 30 normal individuals. β2 –microglobulin gene (β2M) was used as an endogenous reference. Relative changes in TCR ζ chain expression level were used by the 2－ △△Ctmethod between patients with AML and normal individuals. Results In compare with normal individuals groups, the feature of TCR ζ chain expression level could be divided into 3 different groups with absent expression (16%), down expression (26%) and over expression (58%) respectively. Similar expression pattern of TCR ζ chain expression level was found in the 3 subtypes of AML. Conclusions The SYBR GreenⅠ real-time technique for quantitative detection of TCR ζ chain expression levels was established successfully. This is, to our knowledge, the first description in t