乳酸乳球菌质粒pAG712的特性的研究.pdfVIP

现代食品科技 Modern Food Science and Technology Vol.21 No.4(总86) 乳酸乳球菌质粒pAG712的特性研究 1,2 2 2 2 罗立新 , Claire A. Shearman , Tracy Eaton Michael J. Gasson (1.华南理工大学生物科学与工程学院 广东广州 510640) (2.Institute of Food Research, Norwich Research Park, Colney, Norwich, NR4 7UA, UK) 摘要 乳酸乳球菌质粒pAG712 (15.5kb)赋予乳酸乳球菌细胞凝聚表型 它是以è 模式进行复制 复制蛋白与其它以è 模式进行 复制的质粒的复制蛋白具有极高的同源性 复制区上的BglII (4.2kb)片段决定质粒可以在乳酸乳球菌中进行复制 在本研究中 将抗 生素抗性基因插入到质粒pAG712 中 给予它选择性的标记,在研究中发现凝聚基因 aggregation gene 的存在导致了乳酸乳球菌质粒 pAG712 的分离不稳定性 关键词 乳酸乳球菌 质粒 特性 分离 稳定性 凝聚 Characterisation of the Lactococcal Plasmid pAG712 1,2 2 2 2 Luo Li-xin , Claire A. Shearman , Tracy Eaton , Michael J. Gasson 1.College of Bioscience and Engineering, South China University of Technology, Guangzhou 510640, China (2.Institute of Food Research, Norwich Research Park, Colney, Norwich, NR4 7UA, UK Abstract: The lactococcal plasmid pAG712 (15.5kb) confers an aggregation phenotype on the cells. It replicates by a theta mechanism, the replication protein has homology with other theta replication proteins. A BglII fragment (4.2kb) which spans the location of the replication protein allows replication in Lactococcus. In this study, the pAG712 plasmid has been marked with an antibiotic resistance gene to give a selectable marker. And we have demonstrated that the presence of aggregation gene resulted in the segregational instability of pAG712 plasmid. Keywords: Lactococcus lactis; plasmid; characterisation; segregation; stability; aggregation 乳酸乳球菌是在世界范围内得到广泛应用的重 因插入到质粒pAG712 中 给予它选择性的标记 对 要的工业微生物 乳酸乳球菌属的菌种通常含有大量 质粒pAG712 的遗传特性