AssociationofTNF-oLTApolymorphismswithCrohn39;s：TNF基因多态性与克罗恩病和奥尔塔协会的39；.pdfVIP

/locate/issn Cytokine 35 (2006) 13–20 Association of TNF-a/LTA polymorphisms with Crohn’s disease in Koreans Suk-Kyun Yang a, Seong-Gene Lee b, Yun-Kyung Cho a, Jiyoung Lim c, Inchul Lee d, Kyuyoung Song c,* a Department of Internal Medicine, Asan Medical Center, University of Ulsan College of Medicine, Republic of Korea b Department of Biotechnology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, Republic of Korea c Department of Biochemistry and Molecular Biology, University of Ulsan College of Medicine, Seoul 138-736, Republic of Korea d Department of Pathology, University of Ulsan College of Medicine, Seoul, Republic of Korea Received 8 February 2006; received in revised form 28 June 2006; accepted 2 July 2006 Abstract Polymorphisms of the proinflammatory and immunoregulatory cytokines, tumor necrosis factor-a (TNF-a) and lymphotoxin-a (LTA), have been shown to affect their production and be associated with Crohn’s disease. However, the actual alleles associated with the disease are variable among populations. The aim of this study was to test whether TNF-a and LTA polymorphisms were associated with Crohn’s disease risk in Korean samples. Genotyping for five TNF-a promoter polymorphisms (1031, 863, 857, 308, and 238) and two LTA polymorphisms (intron 1 and Thr60Asn) were performed on 289 Korean patients with Crohn’s disease and 399 unrelated healthy controls. Carriers of an individual polymorphism of TNF-a at 857T, showed statistically significant association with Crohn’s disease (adjusted OR = 1.64, 95% CI = 1.11–2.41, P = 0.013). Following haplotype analysis, carriers of the haplotype con