PCR快速检测水产品中副溶血性弧菌的方法研究.pdf

490 2008, Vol. 29, No. 11 食品科学 ※分析检测 1 , 2 1 1 1 刘 琦 ，周德庆 ，柳淑芳 ，苏来金 (1. 中国水产科学研究院黄海水产研究所，山东 青岛 266071 ；2.上海海洋大学食品学院，上海 200090) 为了满足食品卫生快速反应体系的需要，提高水产品中副溶血性弧菌( Vibrio parahaemolyticus，VP) 的检 测效率，本实验针对副溶血性弧菌特异性 t l 基因设计合成引物，采用酚 - 氯仿法提取 D N A ，利用大肠杆菌 (Escherichia coli) 、沙门氏菌(Salmonella) 、志贺氏菌(Shigella) 、溶藻弧菌( Vibrio alginolyticus)做方法特异性对照， 建立了水产品中副溶血弧菌 PCR 快速检测方法。利用此方法对市场随机抽取的 40 份水产品进行检测，并用国家标 准方法(GB/T4789.7 — 2003)对该方法的检验结果进行评价。结果表明：该方法操作简便，特异性强，菌液灵敏 3 度为 10 CFU/ml ，含菌量 1～10CFU/g 的样品经增菌6h 后即可检出。与国标方法相比更加准确可靠，检测周期 10h 即可完成。说明此方法适合水产品中副溶血性弧菌的快速检测。 副溶血性弧菌；水产品；检测 Study on PCR Method for Rapid Detection of Vibrio parahaemolyticus in Aquatic Products 1,2 1 1 1 LIU Qi ，ZHOU De-qing ，LIU Shu-fang ，SU Lai-jin (1.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China； 2. College of Food Science, Shanghai Ocean University, Shanghai 200090, China) Abstract ：In order to improve the detection efficiency of Vibrio parahaemolyticus in aquatic products to meet the need of a system of rapid reaction for food sanitationlas PCR method was established. A primer in connection with t1 gene which is specific for Vibro parahaemolyticus was designed and synthesized, phenol-chloroform method was used to extract DNA, and controls for method specificity were made with Escherichia coli, Salmonella, Shigella, Vibrio alginolyticus. 40 aquatic products sampled at random from market were tested by using this method, and the results were