西兰花中的抗癌成分及其活性的初步研究_王蓉.pdfVIP

※营养卫生 食品科学 2009, Vol. 30, No. 07 243 西兰花中的抗癌成分及其活性的初步研究 王 蓉，何 磊 (济南大学食品科学与营养系，山东济南 250002) 摘 要：目的：本实验对西兰花所含有的抗癌成分进行研究。方法：新鲜西兰花花蕾的甲醇提取物用乙酸乙酯和 n - 丁烯醇萃取后，通过硅胶层析、薄层层析、高效液相等进行分离、提纯，得到化合物A 、B 。然后利用NMR 、 FAB -M S 等仪器分析，确定其结构，并用免疫印迹法测得其抗癌活性。 结果：经过仪器分析，确定这两种抗癌 活性成分为单亚麻酰甘油和单亚油酰甘油，在浓度为140mol/L 和150mol/L 时能抑制Raj i 细胞EB 病毒早期抗原的合 成。 结论：日常食用的绿色蔬菜中含有抗癌活性成分，可以通过日常饮食的摄入达到防癌的目的。 关键词：西兰花；抗癌；活性成分 Primary Study on Anti-tumor Components in Broccoli and Their Bioactivity WANG Rong ，HE Lei (Department of Food Science and Nutrition, University of Jinan, Jinan 250002, China) Abstract ：Obj ective: Broccoli is one of the green vegetables with high nutrition. This study was designed to isolate and analyze the anti-tumor promoters in broccoli. Method: Compounds A and B were isolated from the fresh flower buds of broccoli via the extractions of methanol, EtOAc and n-BuOH and the purifications of silica gel column, thin layer chromatography and high performance liquid chromatography in turn. Then the structures of two compounds were identified by spectroscopic data of NMR and FAB-MS, and the anti-tumor-promoting activity was examined by an immunoblotting analysis. Results: Compounds A, B are identified as monolinolenoylglycerol and monolinoleoylglycerol, respectively, which may inhibit the induction of EBV EA in the early stage at respective concentrations of 140 mol/L and 150 mol/L in the medium. Conclusion: There are anti-tumor promoters in green vegetables, and cancer may be prevented by intake of vegetables. Key words ：broccoli ；anti-tumor ；bioactive component 中图分类号：R 151 文献标识码：A 文章编号：1002-6630(2009)07-024