漆酶在多孔玻璃珠上的固定化研究.pdfVIP

漆酶在多孔玻璃珠上的固定化研究 谢柏盛, 贾红华, 谢迎春，韦萍 （南京工业大学生物与制药工程学院，南京 210009 ） 摘要：本文就漆酶的固定化及固定化酶性质作了初步研究。利用多孔玻璃珠为载体，以3-氨基丙基三 乙氧基硅烷对载体表面进行修饰，再以戊二醛为交联剂，对漆酶进行了固定化。通过对漆酶在多孔玻璃珠 上固定化条件进行优化，获得了最优化固定化条件。结果表明：0.015g 载体中，加入初酶液浓度为0.15 g/L 的漆酶溶液，在pH 为4.4 时，转速170r/min 的摇床中振荡交联5 小时，制得固定化酶。研究发现，固定化 漆酶的最适反应温度为35℃，最适pH 为4.4，在连续操作7 次后，酶的活力仍能保持在51%，固定化漆酶 热稳定性，pH 耐受性，贮存稳定性都明显高于游离漆酶。 关键词：多孔玻璃珠；固定化；漆酶 中图分类号：Q814.2 文献标识码：A Immobilization of laccase on porous glass XIE Bai-sheng,JIA Hong-hua,XIE Ying-chun, WEI Ping Biotechnology and Pharamaceutical Engineering, Nanjing university of technology Nanjing 210009 Abstract : The immobilization of laccase and the properties of the immobilization laccase were studied. Laccase was immobilized by porous glass as the carrier ,and 3- aminopropyltriethoxysilane) were modified on the surface of the carrier, then glutaraldehyde as crosslinking agent. By laccase immobilized on porous glass were optimized to achieve the most optimal conditions of immobilization. The results showed that: 0.015g vector, adding initial enzyme concentration was 0.15 g / L of laccase solution, pH 4.4, the speed of the shaker 170r/min 5 hours in the oscillation of cross-linking, the immobilized enzyme obtained. Study found that the immobilized laccase optimum temperature is 35℃, the optimum pH is 4.4, after 7 times in continuous operation, the enzyme activity still remained at 51%, the thermal stability of immobilized laccase, pH tolerance resistance, storage stability are significantly higher than free laccase. Keywords: porous glass;immobilization;laccase 基金项目：国家自然科学基金。联系人：贾红华，电话：025，E-mail ： hhjia@ 。第一作者：谢柏盛（1986-5），男，学士，在读研究生。 引言 漆酶是一种含铜的多酚氧化酶，可以降解环境污染物中的大量酚类、含氯污染物 和许多致癌的多环芳香化合物[1] ，大量研究表明漆酶有望在环境污染