人牙周韧带细胞和牙髓细胞表型特征的比较.docVIP

人牙周韧带细胞和牙髓细胞表型特征的比较 人牙周韧带细胞和牙髓细胞表型特征的比较 作者：吴莉萍 韦曦 凌均棨 刘路 作者单位：1.中山大学光华口腔医学院·附属口腔医院 正畸科；2.牙体牙髓科，广东 广州 510055 　　【摘要】 目的 研究人牙周韧带细胞（PDLCs）和牙髓细胞（DPCs）表型特征以及体外传代对其表型特征的影响，为选择适宜的表面标记分选生物性状同源性的细胞亚群提供依据。方法 采用酶消化法体外培养PDLCs和DPCs，免疫组化检测和流式细胞仪分析细胞表面标记的表达。结果 PDLCs和DPCs的STRO-1和CD146免疫组化染色阳性。流式细胞仪分析显示：获得的第1代PDLCs和DPCs表达间充质干细胞表面标记STRO-1、CD146、CD29、CD44和CD106，基本不表达CD34。PDLCs的STRO-1、CD29和CD44阳性表达百分比与DPCs间的差异无统计学意义（P0.05），PDLCs的CD106阳性表达百分比高于DPCs，CD146阳性表达百分比低于DPCs，且差异有统计学意义（P0.001）。PDLCs和DPCs表达STRO-1和CD146的阳性表达百分比随传代而逐渐减低。结论 PDLCs表面抗原表达与DPCs相似，而且其中成体干细胞的成分随传代逐渐减少。 　　【关键词】 牙周韧带细胞 牙髓细胞 干细胞 表面抗原 　　The surface antigen expression of periodontal ligament cells and dental pulp cells in vitro WU Li-ping1, WEI Xi2, LING Jun-qi2, LIU Lu2.（1. Dept. of Orthodontics, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou 510055, China; 2. Dept. of Endodontics, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou 510055, China） 　　[Abstract] Objective To investigate the expression of surface antigen of human periodontal ligament cells（PDLCs） and dental pulp cells（DPCs） and the impact of ex vivo expansion to the expression of surface antigen. Toprovide basis of proper surface antigen for further selection of homogenous stem cell subpopulation from PDLCs and DPCs. Methods PDLCs and DPCs were isolated and cultured by collagenase type I and dispase. The expression of surface antigen was analyzed by immunohistochemistry and flow cytometry. Results Positive expression of STRO-1and CD146 were observed in PDLCs and DPCs by immunocytochemistry. Similar to DPCs, PDLCs expressed mesenchymal stem cell markers STRO-1, CD146, CD29, CD44 and CD106, and displayed negative expression for CD34 at passage 1 by flow cytometry. There were no significant difference of STRO-1, CD29 and CD44 expression level between PDLCs and DPCs（P0.05）. PDLCs expressed significantly higher level of CD106 and significantly lowerlevel of CD146 than DPCs（P0.001）. The proportion of STRO