欧美杂种山杨体细胞无性系变异的分析.pdfVIP

植物学通报 2006, 23 (1): 44~51 Chinese Bulletin of Botany . . 实验简报 欧美杂种山杨体细胞无性系变异的分析 1 1* 1 2 詹亚光 齐凤慧 高瑞馨 杨传平 (1 东北林业大学生命科学学院 哈尔滨 150040) (2 东北林业大学林学院 哈尔滨 150040) 摘要 以成年欧美杂种山杨(Pop ulus tremula ×P. tremuloides)优良无性系为材料, 通过组织培养方法获 -1 . 得体细胞无性系, 利用细胞学和分子生物学方法对其发生的变异进行研究。结果表明: 用3.0 mg L 2, 4-D诱导的再生植株的细胞染色体稳定性较差, 所检测的144个细胞中, 变异的细胞中多数发生了染色 体加倍, 二倍体细胞数仅占36.81% 。有些染色体还发生了形态变异, 染色体加长, 形成带有随体或长臂 -1 -1 . . 较长的大型染色体。用1.0 mg L 6-BA诱导再生植株中染色体数量稳定性介于对照和3.0 mg L 2, 4-D 诱导的再生植株之间, 在观察的142个细胞中二倍体细胞占54.93% 。再生植株的AFLP分析表明, 由激素 诱导的再生植株中, AFLP谱带发生了变化, 表明发生了体细胞无性系分子水平变异。 关键词 欧美杂种山杨, 体细胞无性系, 染色体, AFLP Cytological and Molecular Biological Assay of the Somaclonal Variations of Pop ulus tremula ×P. tremuloides 1 1* 1 2 Yaguang Zhan , Fenghui Qi , Ruixin Gao , Chuanping Yang (1 College of Life Science, Northeast Forestry University , Harbin 150040) (2 College of Forest, Northeast Forestry University, Harbin 150040) Abstract The leaves, stems and leafstalks of adult Populus tremula ×P. tremuloides were cultured to generate somaclonal lines, and the variations during culture were assayed by cytological and molecular biological methods. Only 36.81% were diploid in the 144 observed cells from regenerated lines -1 . induced by 3.0 mg L 2, 4-D, which was