量子点免疫荧光技术在病理诊断中的初步应用.pdf

20 2 Vol20No2 2011 4 CHINESE JOURNAL OF HISTOCHEMISTRY AND CYTOCHEMISTRY April2011 1, 3 3 1, 3 3 3 2* 朱小波 黄燕华 彭 俊 高 俊 李蓓芸 陈洪雷 1 2 ( ; ; 3 , 430072) ( quantum dots, QDs) , , QDs LCACalponin p 3 , QDs CK PCNA Her-2 CK QDs LCACalponin p 3 QDs CK PCNA Her-2 CK QDs QDs ; ; ; R392-3 A DOI: 103870/ zgzzhx201102003 Initialapplication of quantum dots immunofluo escence technology in pathological diagnosis 1, 3 3 1, 3 3 3 2* Zhu Xiaobo , Huang Yanhua , Peng Jun , Gao Jun , Li Beiyun , Chen Honglei 1 ( Wuhan T umor N anometer Diagnosis E ngineering Research Center ; 2 Dep artment. of Pathology , Basic Me ical S chool of Wuhan University , Wuhan; 3 Wuhan J iay uan Quantum ots Co. , L TD, Wuhan 430072, China) Abst act Objective Quantum dots ( QDs) immunofluorescence w as compared w ith the immu- noenzyme method in uccurately locating different proteins in paraffin embedded tissues, to detect co-ex- pression of tw o different proteins. T he initial application value of QDS w as investigated by using it. Meth- ods QDs immunofluorescence histochemistry ( QDs-IHC) and immunoenzyme histochemistry were used to detect the expression of p 3 protein in the lung cancer tissues, calponin protein in the myoepithelial cells of breast tissues, and LCA protein in the normal appendix tissues. Co-expression of CK and PCNA proteins in the human cervical intraepithelial neoplasia, Her-2 and CK proteins in the breast cancer tissues w ere simultaneously detected by QDs double- labelin