恰麻古中总黄酮提取工艺.pdfVIP

医药导报2014年7月第33卷第7期 ·935 · 恰麻古中总黄酮提取工艺* 海力茜·陶尔大洪,古娜娜 ·对山别克 (新疆医科大学药学院,乌鲁木齐 830054) 摘 要 目的 通过单因素实验及正交实验探讨提取维药恰麻古中总黄酮的最佳工艺,并测定其不同产地的药材 含量,为资源的合理开发利用提供科学依据。 方法 以乙醇为溶剂回流提取,采用紫外-可见分光光度法(UV)测定恰麻 古中总黄酮的含量,并通过重复性、精密度和回收率实验考察实验方法。 结果 维药恰麻古中总黄酮的最佳工艺为乙醇 浓度75%,提取时间2.5 h,提取温度80 ℃,料液比1 60。 标准液及供试液均在510 nm 波长处有最大吸收,总黄酮在 -1 0.008~0.048 mg ·mL 线性关系良好(r=0.999 3),平均加样回收率99.59%;精密度实验、稳定性实验和重复性实验 的RSD分别为0.022%,1.311%和1.601%,5 个产地的恰麻古中,阿克苏恰麻古的黄酮含量最高,平均含量0.280 2%。 结论 该方法操作简单,重复性好,灵敏度高,适用于恰麻古总黄酮的含量测定。 关键词 恰麻古;总黄酮;分光光度法,紫外可见 中图分类号 R286;R927.1 文献标识码 A 文章编号 1004-0781(2014)07-0935-03 Optimization of the Extraction Process for Total Flavonoids fromBrassica rapa L . TAOERDAHONG H,DUISHANBIEKE G( Collegeof Pharmacy,Xinjiang Medical University,Urumqi830054, China ) ABSTRACT Objective To establish the optimum parameters for the flavonoids extraction from Brassica rapa L. by single-factor andorthogonalexperiments,determinethecontentofflavonoidsinBrassicarapaL.fromdifferentorigins,andprovide ascientificbasisfortherationaldevelopmentandutilizationofBrassicarapaL. Methods ThetotalflavonoidsinBrassicarapa L.was extracted by ethanol reflux extraction and the content was determined by ultraviolet-visible spectrophotometry.The method was validated by tests of stability,precision and recovery. Results The optimum extracting condition for flavonoids from Brassica rapa L.was as follows:using 75% ethanol as extracting agent,setting temperature at 80 ℃,using solid-liquid ratio at 1g 60 mL and extracting for 2.5 h.The detection wavelength was 510 nm.The linear range and the detectable limit of the -1 methodwere0.008-0.048 mg ·mL (r=0.999 3). The average ex