猪肝中维生素B12的检测和提取研究.docVIP

猪肝中维生素B12的检测和提取研究 维生索B12广泛应用于医药、食品和畜牧业，工业化生产维生綮B12主要通过微生物发酵。动物源食品富古维生索B12，如内脏、肝、肾、牛乳及蛋黄大孔树脂吸附法（CAD45）提取维生素样品中的B12 ：维生索B12大孔树脂吸附法Detection and extraction of the liver in vitamin B12 Abstract Objective: Vit B12 is widely used in medicine, food and livestock, industrial production of subsistence embroidered banner B12 mainly through microbial fermentation. Food of animal origin, rich in ancient Vit B12, such as offal, liver, kidney, milk and egg yolk. Understand the amount of vitamin B12 in the liver in the detection of vitamin B12 in the liver in, which can clearly maintain the human body the amount of vitamin B12 uptake of the liver volume. Methods: 1.The atomic absorption method for the determination of the amount of vitamin B12: Vitamin B12 molecules containing cobalt atoms by atomic absorption spectrophotometer can determine the content of cobalt in vitamin B12, vitamin B12 can be obtained through quality conversion. 2. Macroporous resin adsorption (CAD45) extract vitamin samples B12: The Yuming the studies have found that The CAD45 resin at pH 7:00 hydroxy-cobalamin prime has a good adsorption capacity and use 50% ethanol (pH = 2) solution can be used as the eluent, static elution rate of 99% or more . 3.Using high performance liquid chromatography detection of hydroxy-cobalamin amount: By HPLC, using acetonitrile and sodium acetate buffer as the mobile phase to rapid determination of the amount of hydroxy-cobalamin in order to determine the extraction rate of vitamin B12. Keywords: Vit B12 and; hydroxy cobalamin; macroporous resin adsorption; atomic absorption spectrometry; high performance liquid chromatography.  目 录 1 绪论 1 1.1 人工合成维生素B12： 1 1.2 维生素B12的特点： 1 1.2.1 物理特点 2 1.2.2 分子结构特点 2 1.3 维生素B12 生理作用 2 1.4 维生素B12 病理 3 1.5 维生素B12吸收代谢 3 1.6 维生素B12的来源 5 1.6.1 工业废液中提取维生素B12 5 1.6.2 污泥中提取维生素B12 5 1.6.3 微生物纯种发酵 5 1.6.4 混合培养发酵生产 6 2.实验材料与仪器 6 2.1实验材料、试剂 6 2.2 实验仪器 6 3 实验方法和步骤 6 3.1 样品的预处理 6 3.2 原子吸收法测定维生素B12 6 3.2.1 钴的标准溶液配置 6 3.2.2工作曲线的绘制 6 3.2.3 样品的分析 6 3.2.4 数据处