《Effect of lowdose Xray radiation on adaptive response in gastric cancer cell》.pdfVIP

Chinese-German J Clin Oncol April 2013, Vol. 12, No. 4, P171–P174 DOI 10.1007/s10330-012-1134-2 Effect of low-dose X-ray radiation on adaptive response in gastric cancer cell Shukai Wang, Gang Jiang, Hongsheng Yu, Xiangping Liu, Chang Xu Department of Oncology, The Affiliated Hospital of Medical College Qingdao University, Qingdao 266000, China Received: 27 December 2012 / Revised: 28 February 2013 / Accepted: 25 March 2013 © Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg 2013 Abstract Objective: We aimed to study the effect and mechanism of low-dose radiation (LDR) on adaptive response of gastric cancer cell. Methods: SGC7901 cells were cultured in vitro, and divided into 4 groups: control group (D0 group), low-dose radiation group (D1 group, 75 mGy), high-dose radiation group (D2 group, 2 Gy), low-dose plus high-dose radiation group (D1 + D2 group, 75 mGy + 2 Gy, the interval of low and high-dose radiation being 8 h). Cell inhibition rate was detected by cytometry and CCK8 method; the proportion of cell cycle at different times after irradiation was determined by using a flow cytometry. The ATM mRNA levels were detected by using quantitative real-time reverse transcription polymerase chain reac- tion (RT-PCR). Results: There was no significant different between groups D0 and D1, groups D2 and D1 + D2 cell inhibition rate (P 0.05). There was a significant increase G2/M arrest in groups D2 and D1 + D2 than groups D0 and D1 after 6 h of radiation and did not recover at 48 h (P 0.05). The ATM mRNA expression of group D2 and D1 + D2 increased highly than that of group D0 and D1 (P 0.05). However, differences between group D2