《Effects of plant growth regulators carbon sources and pH values on callus induction in Aquilaria malaccensis leaf explants and characteristics of the resultant calli》.pdfVIP

Journal of Forestry Research (2014) 25(3): 535−540 DOI 10.1007/s11676-014-0492-8 ORIGINAL PAPER Effects of plant growth regulators, carbon sources and pH values on callus induction in Aquilaria malaccensis leaf explants and characteris- tics of the resultant calli Shashita JAYARAMAN • Nurul Hazwani DAUD • Rasmina HALIS Rozi MOHAMED Received: 2013-01-11; Accepted: 2013-03-18 © Northeast Forestry University and Springer-Verlag Berlin Heidelberg 2014 Abstract: The endangered tropical tree, Aquilaria malaccensis , produces tively. Microscopic observations revealed the arrangement of the friable agarwood for use in fragrance and medicines. Efforts are currently un- cells as loosely packed with relatively large cells, while for the compact derway to produce valuable agarwood compoundsn tissue culture. The callus, the cells were small and densely packed. We concluded that MS purpose of this study was to develop an optimal growth medium, specif- medium containing 15 g/L sucrose, 1.1 µM NAA + 2.2 µM BAP hor- ically, the best hormone combination for callus suspension culture. Using mone combination, and a pH of 5.7 was highly effective for inducing nursery-grown A. malaccensis , sterilized leaf explants were first incu- friable callus from leaf explants of A. malaccensis for the purpose of bated on basic Murashige and Skoog (MS) gel medium containing 15g/L establishing cell suspension culture. sucrose and at pH 5.7. Different auxin types including Keywords: Agarwood, endangered tree, in vitro, plant growth regulators, 1-naphthaleneacetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D),