《H5N1 Avian Influenza Prepandemic Vaccine Strains in China》.pdfVIP

Biomed Environ Sci, 2014; 27(10): 763-769 763 Original Article H5N1 Avian Influenza Pre-pandemic Vaccine Strains in China* BO Hong, DONG Li Bo, ZHANG Ye, DONG Jie, ZOU Shu Mei, GAO Rong Bao, WANG Da Yan, and SHU Yue Long# National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention; Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing 102206, China Abstract Objective To prepare the 4 candidate vaccine strains of H5N1 avian influenza virus isolated in China. Methods Recombinant viruses were rescued using reverse genetics. Neuraminidase (NA) and hemagglutinin (HA) segments of the A/Xinjiang/1/2006, A/Guangxi/1/2009, A/Hubei/1/2010, and A/Guangdong/1/2011 viruses were amplified by RT-PCR. Multibasic amino acid cleavage site of HA was removed and ligated into the pCIpolI vector for virus rescue. The recombinant viruses were evaluated by trypsin dependent assays. Their embryonate survival and antigenicity were compared with those of the respective wild-type viruses. Results The 4 recombinant viruses showed similar antigenicity compared with wild-type viruses, chicken embryo survival and trypsin-dependent characteristics. Conclusion The 4 recombinant viruses rescued using reverse genetics meet the criteria for classification of low pathogenic avian influenza strains, thus supporting the use of them for the development of seeds and production of pre-pandemic vaccines. Key words: Influenza; H5N1; Pre-pandemic; Vaccine strains Biomed Environ Sci, 2014; 2 7(10): 763-769 doi: 10.