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10.1007/s11771-011-0854-8
Heavymetalsaccumulationeffectin
rabbitbodyfluidsaftersmoking
ZHONGKuang-biao1 GUIMing1 ZHULi-yong1
LIWei1 GUANCha-xiang1 GUOFang-qiu2
1.UrologySurgeryofTheThirdXiangyaHospital,CentralSouthUniversity,Changsha410083,China;
2.SchoolofChemistryandChemicalEngineering,CentralSouthUniversity,Changsha410083,China
Abstract:Concentrationofheavymetalsinbloodandurineofrabbitafterinhalingthreedifferentkindsofcigarettewasstudied
throughtheanimalpassivesmokingpattern.Thesampleswerepreparedbynitricacidsolutiondigestionanddeterminationofseven
kindsofheavymetalsincludingHg,Se,Sn,Pb,Cd,NiandCrwasperformedbyinductivelycoupledplasma-atomicemission
spectrometry(ICP-AES).TheICP-AESmethodwasestablishedwithgoodprecisionandaccuracy,relativestandarddeviation(n=6)
wasbetween2.9% and5.9%,andtherecoverywasintherangeof95.0%-104.2%.Concentrationofsixheavymetalsincreasesin
someextentinbloodandurineafterperiodofsmokingandtheincreasingofheavymetalsinbloodandurineallshowstime
dependence.Significantlyhigherheavymetallevelsareobservedinthebloodandurineofthecigaretteinhalingrabbitsinthe
exposedgroup.Theconcentrationofsixkindsofheavymetalsinthebloodoftherabbitincreasesafter16weeksexposingto
cigarettesmoking.ThreetimesofHg,tentimesofSeandtraceamountofPb,Cd,NiandCraredetectedinthebloodafter16weeks
ofsmoking.Forurinesamples,aboutthreetimesofHg,twotimesofSe,fivetimesofPbandtraceamountofCdaredetectedafter16
weeksofinhalationofcigarette.Comparatively,higherconcentrationofheavymetalsaredetectedafterinhalingofNisecigarette.
inductivelycoupledplasma-atomicemissionspectrometry;blood;urine;heavymetalelement
Project(11JJ5053)supportedbytheProvincialNaturalScienceFoundationofHunanProvince,China
2011-06-02 2011-08-08
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