Purification+and+Identification+of+a+Clotting+Protein+from+the+Hemolymph+of+Chinese+Shrimp+(Fenneropenaeus+chinensis).pdfVIP
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Purification+and+Identification+of+a+Clotting+Protein+from+the+Hemolymph+of+Chinese+Shrimp+(Fenneropenaeus+chinensis).pdf
JIOcean andCoastalSea
Univ.China(OceanicResearch)
DOI
10.1007/s11802—013-2026一Y
ISSN
1672—5182,201312(3):477—483
胁tp.//www.ouc.edu.cn/xbywb/
E-maihxbywb@OUC.edu.cn
a
PurificationandIdentificationof Proteinfrom
Clotting
the ofChinese
HemolymphShrimp
(Fenneropenaeuschinensis)
WANG Guofan¨,
Baojiel),孙,PENGHongni扪,LIUMei¨,JIANGKeyongn,ZHANG
and Leil),+
WANG
26607R.China
1,P
1)InstituteofOceanlogy,ChineseAcademyofSciences,Qingdao
School 1 R.China
2)Graduate 00049,P
ofChineseAcademyofSciences,Beoing
November9,201
(Received8,2012:revisedMay29,2012;accepted 2)
May
of Pressand Berlin 2013
@Ocean China.Science
University Springer-VerlagHeidelberg
anddiverserolesin as and
AbstractThe crustaceans,such
clottingproteinrCP)playsimportant coagulationlipidtransportation.
A was fromthe ofChinese chinensis(namedasFc·CP)with
clottingproteinpurified hemolymph shrimpFenneropenaeus Qsepha—
roseHP and HP interaction wasabletofclrm
anion.exchangechromatographyphenylsepharosehydrophobicchromatography.Fc-CP
stableclotsinvitrointhe ofhemocyteand thatthe reactionis a
presence 1ysate Ca”.suggestingclotting catalyzedby
of 380kDaunder condi
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