Enumeration and viability of rare cells in a microfluidic disk via positive selection approach》.pdfVIP

Analytical Biochemistry 429 (2012) 116–123 Contents lists available at SciVerse ScienceDirect Analytical Biochemistry journal homepage: www.else /locate/yabio Enumeration and viability of rare cells in a microfluidic disk via positive selection approach Ken-Chao Chen a, Yu-Cheng Pan a, Chen-Lin Chen a, Ching-Hung Lin b, Chiun-Sheng Huang c, Andrew M. Wo a,⇑ a Institute of Applied Mechanics, National Taiwan University, Taipei, Taiwan b Department of Oncology, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan c Department of Surgery, National Taiwan University Hospital, College of Medicine, National Taiwan University, Taipei, Taiwan a r t i c l e i n f o a b s t r a c t Article history: Recent studies have shown that specific rare cells in the blood can serve as an indicator of cancer prog- Received 28 February 2012 nosis, among other purposes. This article demonstrates the concept of separating and detecting rare cells Received in revised form 7 June 2012 from peripheral blood mononuclear cells via an economical microfluidic disk with a model system. MCF7, Accepted 3 July 2012 labeled with magnetic beads, was used to simulate circulating tumor cells as a target. Jurkat clone E6-1 Available online 16 July 2012 was used to simulate leukocytes or other cells abundant in human blood. A tailored multistage magnet maximized the magnetic field to ensure optimal trapping efficiency. Results indicate that the yield of Keywords: