Fluorescence enhancement and multiple protein detection in ZnO nanostructure microfluidic devices》.pdfVIP

Biosensors and Bioelectronics 75 (2016) 285–292 Contents lists available at ScienceDirect Biosensors and Bioelectronics journal homepage: /locate/bios Fluorescence enhancement and multiple protein detection in ZnO nanostructure microfluidic devices Chen-Hsiang Sang a, Shu-Jen Chou b, F.M. Pan a, Jeng-Tzong Sheu c,n a Institute of Nanotechnology/Department of Materials Science and Engineering, National Chiao Tung University, 1001 Ta-Hsueh Road, Hsinchu 30050, Taiwan b Institute of Plant and Microbial Biology, Academia Sinica, Taipei 115, Taiwan c Institute of Boimedical Engineering, National Chiao Tung University, 1001 Ta-Hsueh Road, Hsinchu 30050, Taiwan a r t i c l e i n f o a b s t r a c t Article history: In this study, different morphological ZnO nanostructures, those of sharp nanowires (NWs), rod NWs, Received 17 June 2015 and hexahedral-puncheon nanostructures, were grown in microfluidic channels on the same glass Received in revised form substrate. Characterizations of correspondent biomolecule binding properties were simulated and de- 13 August 2015 monstrated. The surface was modified using 3-ammineopropyl-triethoxysilane (3-APTES) and biotin-N- Accepted 21 August 2015 hydroxysuccinimide ester (NHS-biotin). Different concentrations (4.17 pM to 41.7 nM) of dye-conjugated Available online 22 August 2015 streptavidin were simultaneously infused through the second microfluidic channels, which li