ReDuNing, a patented Chinese medicine, reduces the susceptibility to H1N1 influenza of mice loaded with restraint stress》.pdf

Available online at ScienceDirect European Journal of Integrative Medicine 6 (2014) 637–645 Original article ReDuNing, a patented Chinese medicine, reduces the susceptibility to H1N1 influenza of mice loaded with restraint stress Lu-Ping Tang a,1, Zhong-Fu Mao a,1, Xiao-Xiao Li a , Min Chen a , Shan-Bing Li a , Bun Tsoi a , Ling-Fang Cao a , Ling Li a , Ji-Mei Zeng a , Zhi-Wei Wang a , Gui-Yuan Yu a , Wei Xiaob,∗, Yi-Fang Li a,∗, Hiroshi Kurihara a , Rong-Rong He a,∗ a Pharmacy College, Jinan University, Guangzhou 510632, China b State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China Received 1 April 2014; received in revised form 30 July 2014; accepted 30 July 2014 Abstract Introduction: ReDuNing (RDN), a patented traditional antipyretic-detoxicate Chinese medicine, has been widely applied as an anti-inflammatory and anti-infectious drug in Chinese clinics. However, relative experimental data on anti-influenza virus and its subsequent viral pneumonia by RDN are limited. In this study, restraint stress mice model, which could mimic the physiological conditions of the population susceptible to influenza infection, was applied to investigate the protective effect of RDN against influenza. Methods: Mice were infected with H1N1 influenza virus 3 days after restraint, and RDN was administered to mice the day after stress by intraperitoneal injection for 7 days. Body weight and mortality were recorded every day. Histopathologic changes, lung index, nucleoprotein (NP) gene ex