Relief of delayed oxidative stress by ascorbic acid can suppress radiation-induced cellular senescence in mammalian fibroblast cells》.pdf

Mechanisms of Ageing and Development 146–148 (2015) 65–71 Contents lists available at ScienceDirect Mechanisms of Ageing and Development j o u rn al h omepage: w w w. elsev /locate/mechagedev Relief of delayed oxidative stress by ascorbic acid can suppress radiation-induced cellular senescence in mammalian fibroblast cells Shinko Kobashigawa a,∗ , Genro Kashino b , Hiromu Mori a , Masami Watanabe c a Department of Radiology, School of Medicine, Oita University, Oita, Japan b Advanced Molecular Imaging Center, School of Medicine, Oita University, Oita, Japan c Radiation Biology Center, Kyoto University, Kyoto, Japan a r t i c l e i n f o a b s t r a c t Article history: Ionizing radiation-induced cellular senescence is thought to be caused by nuclear DNA damage that Received 24 December 2014 cannot be repaired. However, here we found that radiation induces delayed increase of intracellular Received in revised form 6 April 2015 oxidative stress after irradiation. We investigated whether the relief of delayed oxidative stress by ascor- Accepted 4 May 2015 bic acid would suppress the radiation-induced cellular senescence in Syrian golden hamster embryo Available online 6 May 2015 (SHE) cells. We observed that the level of oxidative stress was drastically increased soon after irradiation, then declined to the level in non-irradiated cells, and increased again with a peak on day 3 after irradi- Keywords: