乙酰基转移酶KAT2A调控牙周膜干细胞成骨分化的研究.pdfVIP

2015 9 （6 3 ） Orai Biomedicine ，Voi．6 ，No．3，Sep．2015 · 155· ［］ 1674-8603（2015）03-0155-05 KAT2A * ， ，，，， ，， （，510140） ［］ ：（PDLSCs）KAT2A ， KAT2A PDLSCs 。 ：PDLSCs； PDLSCs KAT2A ；、PDLSCs KAT2A PDLSCs 。 ：PDLSCs ，PDLSCs KAT2A ， （P ＜0．05）；KAT2A ，PDLSCs ，（P ＜0．05 ）。 ：PDLSCs KAT2A ，。 ［］ ；；； ［］ R780．2 ［］ A ［doi］ 10．3969／j．issn．1674-8603．2015．03．008 Study of acetyltransferase KAT2A regulating osteogenic differentiation potential ofperiodontal ligament stem cells YUAN Lin ，SUN Jin ，YANG Zhengyi ，CA O Yina ，PAN Guangsi ，QIAN Jun ，HE Enliang ，WANG Han．（Dep artment of Stomatology ，the First Aff iliated Hosp ital of Guangzhou Medical University ，Guangzhou 510140 ，China．） Corresp onding author ：YUAN Lin ，E-mail ：yuanlin00＠hotmail．com ，Tel ：0086 ［Abstract ］ Objective ：To compare the ievei of acetyitransferase KAT2A in periodontai iigament stem ceiis derived from heaithy indi - viduais （H-PDLSCs） with periodontai iigament stem ceiis derived from periodontitis individuai （P-PDLSCs），and to determine the effect of acetyitransferase KAT2A on osteogenic differentiation potentiai of PDLSCs ．Methods ：Human PDLSCs were cuitured and cioned with iimited diiuted method．Quantitative RT-PCR and Western Biot were used to examine different expression of acetyitransferase KAT2A in two hinds of PDLSCs．Quantitative RT-PCR，Western Biot and Aiizarin Red Staining were used to determine osteogenic dif - ferentiation potentiai of PDLSCs with gene KAT2A hnochdown．Results ：Quantitative RT-PCR and Western Biot showed that iower ex - pression of KAT2A was detected in P-PDLSCs compared with that of H-PDLSCs，with statisticai significance（P ＜0．05）．Quantitative RT-PCR，Western Biot and Aiizarin Red Staining indicated that osteogenic differentiation potentiai was inhibited in H -PDLSCs with KAT2A hnochdown and the effect was statisticaiiy significant （P ＜0．05 ）．Conclusions ：