Expression and Analysis of Microtus fortis against Schistosoma japonicum CD36 Gene-论文.pdfVIP
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Expression and Analysis of Microtus fortis against Schistosoma japonicum CD36 Gene-论文.pdf
August2013,Vo1.7,No.8,PP.791·795
JournalofLifeSciences,ISSN 1934-7391,USA
ExpressionandAnalysisofMicrotusfortisagainst
SchistosomajaonicumCD36Gene
YangXiang,Xue—QinZheng,ZhenXiang,Dong.SongNie,YuLiu,Xian.LeiLi,Dian-DianLv,Jun-Jian
Hu2andPeiHe2
J.CollegeofChemistryandChemicalEngsineering,HunanInstituteofScienceandTechnology,Yueyang414006,China
2.HunanProvincialInstituteofSchistosomiasisControl,Yueyang414006,China
Received:December19,2012/Accepted:July22,2013/Published:August30,2013
Abstract:ThetotalRNAwasextractedfromMicrotusfortislivertissuewhichbeforebeinginfectedandafterbeinginfected10d
and 15dbytheSchistosomajaponicum cercariae.UsingRattusnorvegicusCD36geneprobetohybridizenaalysisofCD36
differenceexpressionintheMicrotusfortis1ivertissueswhichwereinfectedwithSchistosomajaponicumbeforeandafterbeing
infected.Atthesametime,thecDNA sequencena dencodedaminoacidsequenceofhteRattusnorvegicusCD36genenadCD36
proteinstructuraldomainswerena alysizedbyusingbioinformatics.TheresultsshowedthattheCD36expressionlevelsinthe1iver
tissueofMicrotusfortisafterbeinginfectedweresignificnatlyhigherthna beforebeinginfectied.TheRattusnorvegicusCD36
eDNA sequenceofatotallengthis1625bpna dencoded472ma inoacidresiduesandRattusnorvegicusCD36proteincontaininga
CD36superfamilydomain.
Keywords:Microtusfortis,Schistosomajaponicum,CD36,expression,naalysis
1.IntrOductiOn schistosomiasis chraacteristics 【1】. Oriental vole
distribution in China ismainly concentrated in the
Schistosomiasis is the world’s second—largest
YangtzeRiverBasin,especially in endemicarea of
parasiticdiseaseaftermalaria,andisseriousharm to
schistosomiasisjaponicainDongtingLkaeIsland,isa
humanhealth.Atleast76countriesandregionstobe
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