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GPR49基因对肝癌细胞增殖侵袭能力的影响.pdf
第 6卷 第 3期 器官移植 Vo1.6 No.3
2015年 5月 OrganTransplantation Mav.2015
· 实 验 研 究 ·
GPR49基因对肝癌细胞增殖侵袭能力的影响
汤凯雯 唐云强 龚远锋 赵宏宇 王嘉康 唐辉 麦聪
【摘要】 目的 探讨肝癌细胞系Huh7细胞中G蛋白偶联受体49(GPR49)基因对肝癌细胞侵
袭能力的影响及其分子生物学机制。方法 根据转染的小干扰RNA (si—RNA)不同,将 Huh7细胞分
为GPR49一siRNA (si—GPR49)组和阴性对照 NC-siRNA (si—NC)组 ,另设未转染 Huh7细胞为对照组
(control组)。采用逆转录聚合酶链反应 (RTP·CR)法和Westernblot法分别检测3组细胞GPR49、细
胞周期素D1(cycl
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