《APPLE POMACE》.pdfVIP

APTEFF, 39, 1-212 (2008) UDC: 634.11:543.645 DOI: 10.2298/APT0839201S BIBLID: 1450-7188 (2008) 39, 201-212 Original scientific paper ANTIOXIDANT AND ANTIPROLIFERATIVE ACTIVITY OF GRANNY SMITH APPLE POMACE Slađana M. Savatović, Gordana S. Ćetković, Sonja M. Đilas, Vesna T. Tumbas, Jasna M. Čanadanović-Brunet, Dragana D. Četojević-Simin and Anamarija I. Mandić Granny Smith apple pomace was subjected to evaluation as valuable source of anti- oxidant and anticancer phytochemicals on the basis of its content in phenolic compounds, antioxidant and antiproliferative activity. The total cotent of phenolics, flavonoids and flavan-3-ols in apple pomace determined spectrophotometrically, was 7.02 mg/g, 0.51 mg/g and 8.80 mg/g. Major phenolics (phenolic acids, flavan-3-ols, flavonoids and di- hydrochalcons) in apple pomace were identified and quantified by HPLC. The antioxi- dant activity of apple pomace on stable 1,1-diphenyl-2-picrylhydrazyl (DPPH) and reac- tive hydroxyl radicals, was investigated by electron spin resonance (ESR) spectroscopy. The IC50DPPH and IC50OH values of Granny Smith apple pomace were 9.51 mg/ml and 29.17 mg/ml, respectively. The antiproliferative activities of apple pomace on cervix epi- theloid carcinoma (HeLa), colon adenocarcinoma (HT-29) and breast adenocarcinoma (MCF7) cell lines were determined according to the MTT (3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide) colorimetric assay. The IC50HeLa, IC50HT-29 and IC50MCF7 values of Granny Smith apple pomace were 26.40 mg/ml, 22.47 mg/ml and 21.26 mg/ml, respectively. The significant correlations between antioxidant ac